Assessment of MLL methyltransferase gene expression in larynx carcinoma

Figueiredo,David Livingstone Alves; Rabello,Doralina do Amaral; Zanette,Dalila Luciola; Saggioro,Fabiano Pinto; Mamede,Rui Celso Martins; Zago,Marco Antonio; da Silva,Wilson Araújo; Saldanha-Araújo,Felipe; Pittella Silva,Fabio

doi:10.3892/or.2015.3756

Assessment of MLL methyltransferase gene expression in larynx carcinoma

Authors:
- David Livingstone Alves Figueiredo
- Doralina do Amaral Rabello
- Dalila Luciola Zanette
- Fabiano Pinto Saggioro
- Rui Celso Martins Mamede
- Marco Antonio Zago
- Wilson Araújo da Silva
- Felipe Saldanha-Araújo
- Fabio Pittella Silva
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Affiliations: Laboratory of Molecular Pathology of Cancer, Faculty of Health Sciences, University of Brasília, Campus Universitário Darcy Ribeiro, Brasília, DF 70.910-900, Brazil, Center for Cell Therapy and Regional Blood Center, Medical School of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP 14.048-900, Brazil, Department of Internal Medicine, Medical School of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP 14.048-900, Brazil
Published online on: January 28, 2015 https://doi.org/10.3892/or.2015.3756
Pages: 2017-2022

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Abstract

Larynx cancer is the second most common type of cancer among all head and neck cancers. Deregulation of epigenetic effectors, including altered expression of histone methyltransferases from the MLL (mixed lineage leukemia) family, have been reported in many cancer types, yet little is known concerning their involvement in larynx cancer. Our objective was to determine the expression profile of MLL genes in larynx carcinoma and normal adjacent tissues and correlate this profile to tumor characteristics. We analyzed the expression profile of 5 MLL genes in 13 cases of larynx carcinoma and their adjacent non-tumor tissues using quantitative real-time PCR. MLL3 was significantly downregulated in tumor samples compared to their normal counterparts, and all MLL genes showed decreased expression in advanced tumors compared to tumors in the initial stage. Altered expression in a single MLL gene was associated with a similar alteration in the other MLL genes, revealing a strong correlation of expression in each individual patient. In conclusion, MLL genes may have similar transcriptional control, and decreased expression of these genes may contribute to larynx cancer progression.

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