Screening of differential microRNA expression in gastric signet ring cell carcinoma and gastric adenocarcinoma and target gene prediction

  • Authors:
    • Jian Chen
    • Di Sun
    • Hongjin Chu
    • Zhaohua Gong
    • Chenglin Zhang
    • Benjiao Gong
    • Yan Li
    • Ning Li
    • Lixin Jiang
  • View Affiliations

  • Published online on: April 28, 2015     https://doi.org/10.3892/or.2015.3935
  • Pages: 2963-2971
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Gastric signet ring cell carcinoma (GSRCC) is a unique pathological type of gastric carcinoma that is extremely invasive and has a poor prognosis after diagnosis. The expression of microRNAs has been closely linked to the carcinogenesis of gastric cancer and has been considered as a powerful prognostic marker. Distinctive expression of miRNAs in GSRCC was investigated in the present study. Samples of GSRCC were compared to that of intestinal gastric adenocarcinoma using Agilent microarray technique, and two differentially expressed miRNAs were identified, hsa-miR-665 and hsa-miR‑95. qRT-PCR verification showed downregulation of both miRNAs in signet ring cell carcinoma and upregulation in gastric adenocarcinoma, which was not consistent with the results obtained by the microarray. Target gene prediction using online databases conferred two strong candidate genes, GLI2 and PLCG1. GO/KO analysis of these two genes showed close correlations with carcinogenesis and chemoresistance. It was concluded that hsa-miR-665 and hsa-miR-95 were downregulated in GSRCC but upregulated in intestinal gastric adenocarcinoma, and the relatively differential expression of the miRNAs negatively controlling their target genes could be closely related to the high invasive metastasis and chemoresistance of GSRCC.
View Figures
View References

Related Articles

Journal Cover

June-2015
Volume 33 Issue 6

Print ISSN: 1021-335X
Online ISSN:1791-2431

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Chen J, Sun D, Chu H, Gong Z, Zhang C, Gong B, Li Y, Li N and Jiang L: Screening of differential microRNA expression in gastric signet ring cell carcinoma and gastric adenocarcinoma and target gene prediction. Oncol Rep 33: 2963-2971, 2015
APA
Chen, J., Sun, D., Chu, H., Gong, Z., Zhang, C., Gong, B. ... Jiang, L. (2015). Screening of differential microRNA expression in gastric signet ring cell carcinoma and gastric adenocarcinoma and target gene prediction. Oncology Reports, 33, 2963-2971. https://doi.org/10.3892/or.2015.3935
MLA
Chen, J., Sun, D., Chu, H., Gong, Z., Zhang, C., Gong, B., Li, Y., Li, N., Jiang, L."Screening of differential microRNA expression in gastric signet ring cell carcinoma and gastric adenocarcinoma and target gene prediction". Oncology Reports 33.6 (2015): 2963-2971.
Chicago
Chen, J., Sun, D., Chu, H., Gong, Z., Zhang, C., Gong, B., Li, Y., Li, N., Jiang, L."Screening of differential microRNA expression in gastric signet ring cell carcinoma and gastric adenocarcinoma and target gene prediction". Oncology Reports 33, no. 6 (2015): 2963-2971. https://doi.org/10.3892/or.2015.3935