Time-dependent release of extracellular vesicle subpopulations in tumor CABA I cells

  • Authors:
    • Ilaria Giusti
    • Marianna Di Francesco
    • Laura Cantone
    • Sandra D'Ascenzo
    • Valentina Bollati
    • Gaspare Carta
    • Vincenza Dolo
  • View Affiliations

  • Published online on: August 12, 2015     https://doi.org/10.3892/or.2015.4199
  • Pages: 2752-2759
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Abstract

Investigations into extracellular vesicles (EVs) have significantly increased since their role in physiological and pathological processes has become more clearly understood. Furthermore, it has become increasingly clear that several subpopulations of EVs exist, such as exosomes (EXOs) and microvesicles (MVs). Various methods and techniques used to identify and isolate the specific EVs subpopulations exist. However, these methods should be further elucidated. A deep understanding of the different factors that affect the EVs release may therefore be useful for the standardization of protocols and to establish guidelines for a more adequate analysis and correct inter‑laboratory comparison. In the present study, we investigated whether composition and molecular features of EVs altered over time following a trigger stimulus. Starved CABA I cells were stimulated with FBS and conditioned medium was collected after different time intervals (30 min and 4, 8 and 18 h). The dynamic of EVs release was time-dependent, as shown by the results of scanning electron microscopy. Additionally, the time elapsed from the stimulus affected the size distribution (as highlighted by transmission electron microscopy and NanoSight assay), amount (in terms of the number of particles and protein amount) and molecular composition (CD63, HLA, Ago-2, gelatinases, and plasminogen activators) suggesting that, different EVs subpopulations were released at different time intervals following cell stimulation. Collectively, the results suggested that, parameters useful to standardize procedures for EVs isolation, including stimulation time should be considered.
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November-2015
Volume 34 Issue 5

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Giusti I, Di Francesco M, Cantone L, D'Ascenzo S, Bollati V, Carta G and Dolo V: Time-dependent release of extracellular vesicle subpopulations in tumor CABA I cells. Oncol Rep 34: 2752-2759, 2015
APA
Giusti, I., Di Francesco, M., Cantone, L., D'Ascenzo, S., Bollati, V., Carta, G., & Dolo, V. (2015). Time-dependent release of extracellular vesicle subpopulations in tumor CABA I cells. Oncology Reports, 34, 2752-2759. https://doi.org/10.3892/or.2015.4199
MLA
Giusti, I., Di Francesco, M., Cantone, L., D'Ascenzo, S., Bollati, V., Carta, G., Dolo, V."Time-dependent release of extracellular vesicle subpopulations in tumor CABA I cells". Oncology Reports 34.5 (2015): 2752-2759.
Chicago
Giusti, I., Di Francesco, M., Cantone, L., D'Ascenzo, S., Bollati, V., Carta, G., Dolo, V."Time-dependent release of extracellular vesicle subpopulations in tumor CABA I cells". Oncology Reports 34, no. 5 (2015): 2752-2759. https://doi.org/10.3892/or.2015.4199