Resveratrol induces cell cycle arrest in human gastric cancer MGC803 cells via the PTEN‑regulated PI3K/Akt signaling pathway

  • Authors:
    • Xiaoping Jing
    • Weiwei Cheng
    • Shiying Wang
    • Pin Li
    • Li He
  • View Affiliations

  • Published online on: November 3, 2015     https://doi.org/10.3892/or.2015.4384
  • Pages: 472-478
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Abstract

Resveratrol is a polyphenolic compound that is extracted from Polygonum cuspidatum and is used in traditional Chinese medicine. Previous data have shown that resveratrol inhibits the growth of human gastric cancer. MTT [3-(4,5-dimethylthiazol-2-yl)‑2,5-diphenyl tetrazolium bromide] and trypan blue assays showed that resveratrol significantly decreased the survival rate of MGC803 cells in a concentration‑ and time‑dependent manner. Our flow cytometric analysis showed that resveratrol treatment arrested the cells at the G0/G1 phase of the cell cycle. Furthermore, western blotting demonstrated that resveratrol decreased the protein expression of phospho‑glycogen synthase kinase 3β (p-GSK3β), cyclin D1, phospho‑phosphatase and tensin homologue (p-PTEN), phospho‑phosphatidylinositol 3'‑OH kinase (p-PI3K), and phospho‑protein kinase B (p-PKB/Akt). We also found that resveratrol inhibited the progression of the cell cycle in MGC803 cells by repressing p‑PI3K and p‑Akt expression. Meanwhile, resveratrol did not decrease the phosphorylation level of Akt when the PTEN gene expression was knocked down by an siRNA in the MGC803 cells. Taken together, these results suggest that resveratrol induced cell cycle arrest in human gastric cancer MGC803 cells by regulating the PTEN/PI3K/Akt signaling pathway.
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January-2016
Volume 35 Issue 1

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Jing X, Cheng W, Wang S, Li P and He L: Resveratrol induces cell cycle arrest in human gastric cancer MGC803 cells via the PTEN‑regulated PI3K/Akt signaling pathway. Oncol Rep 35: 472-478, 2016
APA
Jing, X., Cheng, W., Wang, S., Li, P., & He, L. (2016). Resveratrol induces cell cycle arrest in human gastric cancer MGC803 cells via the PTEN‑regulated PI3K/Akt signaling pathway. Oncology Reports, 35, 472-478. https://doi.org/10.3892/or.2015.4384
MLA
Jing, X., Cheng, W., Wang, S., Li, P., He, L."Resveratrol induces cell cycle arrest in human gastric cancer MGC803 cells via the PTEN‑regulated PI3K/Akt signaling pathway". Oncology Reports 35.1 (2016): 472-478.
Chicago
Jing, X., Cheng, W., Wang, S., Li, P., He, L."Resveratrol induces cell cycle arrest in human gastric cancer MGC803 cells via the PTEN‑regulated PI3K/Akt signaling pathway". Oncology Reports 35, no. 1 (2016): 472-478. https://doi.org/10.3892/or.2015.4384