Liver epithelial cells inhibit proliferation and invasiveness of hepatoma cells

Jeng,Kuo-Shyang; Jeng,Chi-Juei; Jeng,Wen-Juei; Sheen,I-Shyan; Li,Shih-Yun; Hung,Zih-Hang; Hsiau,Hsin-I; Yu,Ming-Che; Chang,Chiung-Fang

doi:10.3892/or.2015.4478

Liver epithelial cells inhibit proliferation and invasiveness of hepatoma cells

Authors:
- Kuo-Shyang Jeng
- Chi-Juei Jeng
- Wen-Juei Jeng
- I-Shyan Sheen
- Shih-Yun Li
- Zih-Hang Hung
- Hsin-I Hsiau
- Ming-Che Yu
- Chiung-Fang Chang
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Affiliations: Department of Surgery, Far Eastern Memorial Hospital, New Taipei City, Taiwan, R.O.C., Graduate Institute of Clinical Medicine, National Taiwan University, Taipei, Taiwan, R.O.C., Department of Hepato-Gastroenterology, Chang-Gung Memorial Hospital, LinKou Medical Center, Chang-Gung University, Taipei, Taiwan, R.O.C., Department of Medical Research, Far Eastern Memorial Hospital, New Taipei City, Taiwan, R.O.C.
Published online on: December 8, 2015 https://doi.org/10.3892/or.2015.4478
Pages: 1622-1628

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Abstract

Hepatocellular carcinoma (HCC) is a worldwide malignancy with poor prognosis. Liver progenitors or stem cells could be a potential therapy for HCC treatment since they migrate toward tumors. Rat liver epithelial (RLE) cells have both progenitor and stem cell-like properties. Therefore, our study elucidated the therapeutic effect of RLE cells in rat hepatoma cells. RLE cells were isolated from 10-day old rats and characterized for stem cell marker expression. RLE cells and rat hepatoma cells (H4-IIE-C3 cells) were co-cultured and divided into four groups with different ratios of RLE and hepatoma cells. Group A had only rat hepatoma cells as a control group. The ratios of rat hepatoma and RLE cells in group B, C and D were 5:1, 1:1 and 1:5, respectively. Effective inhibition of cell proliferation and migration was found in group D when compared to group A. There was a significant decrease in Bcl2 expression and increase in late apoptosis of rat hepatoma cells when adding more RLE cells. RLE cells reduced cell proliferation and migration of rat hepatoma cells. These results suggested that RLE cells could be used as a potential cell therapy.

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