Overexpressed genes associated with hormones in terminal ductal lobular units identified by global transcriptome analysis: An insight into the anatomic origin of breast cancer

  • Authors:
    • Jianmin Yang
    • Haijing Yu
    • Liang Zhang
    • Hua Deng
    • Qi Wang
    • Wenping Li
    • Anqin Zhang
    • Hongyi Gao
    • Aihua Yin
  • View Affiliations

  • Published online on: December 28, 2015     https://doi.org/10.3892/or.2015.4523
  • Pages: 1689-1695
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Abstract

Although human breast ducts and terminal ductal lobular units (TDLUs) share the same cell types, ample evidence shows that TDLUs are the predominant site for the origin of breast cancer. Yet, there is still limited information concerning the molecular mechanisms. Analysis of transcriptomic profiles in TDLUs may provide insight into early breast tumorigenesis. We compared genome-wide expression profiles of 8 matched sets of breast main duct and TDLU samples, using significance analysis of microarray (SAM) software to screen differentially expressed genes (DEGs) with fold-change >2.0 and q-value <0.05. Moreover, we used Gene Ontology for functional enrichment analysis. We identified 472 DEGs between the two tissue types, and confirmed 17 randomly chosen DEGs by quantitative reverse transcription-PCR (qRT-PCR). Notably, hormone-related pathways were highly enriched in the TDLU samples, including various hormone-related DEGs that are associated with breast carcinogenesis and tumor progression. Oncogenic upregulation in TDLUs indicates a potential inappropriate or excessive response to successive hormone stimulus during the proliferation, differentiation and lactation cycles of the human mammary gland. Imbalanced hormone reactions may finally result in the early onset of neoplastic transformation that occurs mostly in breast TDLUs.
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March-2016
Volume 35 Issue 3

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Yang J, Yu H, Zhang L, Deng H, Wang Q, Li W, Zhang A, Gao H and Yin A: Overexpressed genes associated with hormones in terminal ductal lobular units identified by global transcriptome analysis: An insight into the anatomic origin of breast cancer. Oncol Rep 35: 1689-1695, 2016.
APA
Yang, J., Yu, H., Zhang, L., Deng, H., Wang, Q., Li, W. ... Yin, A. (2016). Overexpressed genes associated with hormones in terminal ductal lobular units identified by global transcriptome analysis: An insight into the anatomic origin of breast cancer. Oncology Reports, 35, 1689-1695. https://doi.org/10.3892/or.2015.4523
MLA
Yang, J., Yu, H., Zhang, L., Deng, H., Wang, Q., Li, W., Zhang, A., Gao, H., Yin, A."Overexpressed genes associated with hormones in terminal ductal lobular units identified by global transcriptome analysis: An insight into the anatomic origin of breast cancer". Oncology Reports 35.3 (2016): 1689-1695.
Chicago
Yang, J., Yu, H., Zhang, L., Deng, H., Wang, Q., Li, W., Zhang, A., Gao, H., Yin, A."Overexpressed genes associated with hormones in terminal ductal lobular units identified by global transcriptome analysis: An insight into the anatomic origin of breast cancer". Oncology Reports 35, no. 3 (2016): 1689-1695. https://doi.org/10.3892/or.2015.4523