All-trans retinoic acid enhances bystander effect of suicide gene therapy in the treatment of breast cancer

Kong,Heng; Liu,Xia; Yang,Liucheng; Qi,Ke; Zhang,Haoyun; Zhang,Jingwen; Huang,Zonghai; Wang,Hongxian

doi:10.3892/or.2015.4535

All-trans retinoic acid enhances bystander effect of suicide gene therapy in the treatment of breast cancer

Authors:
- Heng Kong
- Xia Liu
- Liucheng Yang
- Ke Qi
- Haoyun Zhang
- Jingwen Zhang
- Zonghai Huang
- Hongxian Wang
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Affiliations: Department of Thyroid and Breast Surgery, Shenzhen Key Laboratory for Endogenous Infection, Shenzhen Nanshan District People's Hospital (The Sixth People's Hospital of Shenzhen), Shenzhen, Guangdong 518052, P.R. China, Department of Human Resource, Shenzhen Nanshan District People's Hospital, Shenzhen, Guangdong 518052, P.R. China, Department of General Surgery, Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong 510282, P.R. China, Clinical Laboratory, Shenzhen Nanshan District People's Hospital, Shenzhen, Guangdong 518052, P.R. China
Published online on: December 29, 2015 https://doi.org/10.3892/or.2015.4535
Pages: 1868-1874

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Abstract

All-trans retinoic acid (ATRA) has been shown to enhance the expression of connexin 43 (Cx43) and the bystander effect (BSE) in suicide gene therapy. These in turn improve effects of suicide gene therapies for several tumor types. However, whether ATRA can improve BSE remains unclear in suicide gene therapy for breast cancer. In the present study, MCF-7, human breast cancer cells were treated with ATRA in combination with a VEGFP-TK/CD gene suicide system developed by our group. We found that this combination enhances the efficiency of cell killing and apoptosis of breast cancer by strengthening the BSE in vitro. ATRA also promotes gap junction intercellular communication (GJIC) in MCF-7 cells by upregulation of the connexin 43 mRNA and protein in MCF-7 cells. These results indicate that enhancement of GJIC by ATRA in suicide gene system might serve as an attractive and cost-effective strategy of therapy for breast cancer cells.

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