|Early detection of peritoneal dissemination of gastrointestinal cancers by reverse-transcriptase polymerase chain reaction|
Authors: T Fujimura, Y Yonemura, I Ninomiya, K Miwa, I Miyazaki, Y Endo, N Ishikawa, T Obata, M Tanaka, T Sasaki
Affiliations: KANAZAWA UNIV,CANC RES INST,KANAZAWA,ISHIKAWA 920,JAPAN.
We examined the usefulness of the reverse-transcriptase polymerase chain reaction (RT-PCR) analysis for E-cadherin mRNA in the early diagnosis of peritoneal dissemination of gastric and colorectal cancers. The RT-PCR analysis was performed on RNA samples extracted from cells which were collected preoperatively from ascites or intraperitoneally infused fluid. E-cadherin mRNA was detected in 2/2 cases (100%) graded into P-1 peritoneal dissemination, indicating the presence of metastatic cells. In the cases with P-2 and P-3 dissemination the gene expression was detected in 2/3 (67%) and 3/5 (60%), respectively. The RT-PCR analysis was more sensitive for P-1 dissemination than cytological examination. These results suggest that determination of E-cadherin gene expression is useful for the early detection of peritoneal dissemination in gastrointestinal cancer because of its high sensitivity and specificity.