Early detection of peritoneal dissemination of gastrointestinal cancers by reverse-transcriptase polymerase chain reaction

  • Authors: T Fujimura, Y Yonemura, I Ninomiya, K Miwa, I Miyazaki, Y Endo, N Ishikawa, T Obata, M Tanaka, T Sasaki
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  • Published online on: Monday, September 1, 1997
  • Pages: 1015-1019
  • DOI: 10.3892/or.4.5.1015

Abstract

We examined the usefulness of the reverse-transcriptase polymerase chain reaction (RT-PCR) analysis for E-cadherin mRNA in the early diagnosis of peritoneal dissemination of gastric and colorectal cancers. The RT-PCR analysis was performed on RNA samples extracted from cells which were collected preoperatively from ascites or intraperitoneally infused fluid. E-cadherin mRNA was detected in 2/2 cases (100%) graded into P-1 peritoneal dissemination, indicating the presence of metastatic cells. In the cases with P-2 and P-3 dissemination the gene expression was detected in 2/3 (67%) and 3/5 (60%), respectively. The RT-PCR analysis was more sensitive for P-1 dissemination than cytological examination. These results suggest that determination of E-cadherin gene expression is useful for the early detection of peritoneal dissemination in gastrointestinal cancer because of its high sensitivity and specificity.
Journal Cover

September 1997
Volume 4 Issue 5

Print ISSN: 1021-335X
Online ISSN:1791-2431

2012 Impact Factor: 2.191
Ranked #33/202 Oncology
(total number of cites)

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APA
Fujimura, T., Yonemura, Y., Ninomiya, I., Miwa, K., Miyazaki, I., Endo, Y., Ishikawa, N., Obata, T., Tanaka, M., & Sasaki, T. (1997). Early detection of peritoneal dissemination of gastrointestinal cancers by reverse-transcriptase polymerase chain reaction. Oncology Reports, 4(5), 1015-1019.
MLA
Fujimura, Yonemura, Ninomiya, Miwa, Miyazaki, Endo, Ishikawa, Obata, Tanaka, and T Sasaki. "Early detection of peritoneal dissemination of gastrointestinal cancers by reverse-transcriptase polymerase chain reaction." Oncology Reports Oncology Reports 4.5 (1997): 1015-1019.
Chicago
Fujimura, Yonemura, Ninomiya, Miwa, Miyazaki, Endo, Ishikawa, Obata, Tanaka, and T Sasaki. "Early detection of peritoneal dissemination of gastrointestinal cancers by reverse-transcriptase polymerase chain reaction." Oncology Reports Oncology Reports 4 no. 5 (1997): 1015-1019.