Early detection of peritoneal dissemination of gastrointestinal cancers by reverse-transcriptase polymerase chain reaction

  • Authors:
    • T Fujimura
    • Y Yonemura
    • I Ninomiya
    • K Miwa
    • I Miyazaki
    • Y Endo
    • N Ishikawa
    • T Obata
    • M Tanaka
    • T Sasaki
  • Corresponding author:
  • View Affiliations

  • Published online on: Monday, September 1, 1997
  • Pages: 1015-1019 DOI: 10.3892/or.4.5.1015

Abstract

We examined the usefulness of the reverse-transcriptase polymerase chain reaction (RT-PCR) analysis for E-cadherin mRNA in the early diagnosis of peritoneal dissemination of gastric and colorectal cancers. The RT-PCR analysis was performed on RNA samples extracted from cells which were collected preoperatively from ascites or intraperitoneally infused fluid. E-cadherin mRNA was detected in 2/2 cases (100%) graded into P-1 peritoneal dissemination, indicating the presence of metastatic cells. In the cases with P-2 and P-3 dissemination the gene expression was detected in 2/3 (67%) and 3/5 (60%), respectively. The RT-PCR analysis was more sensitive for P-1 dissemination than cytological examination. These results suggest that determination of E-cadherin gene expression is useful for the early detection of peritoneal dissemination in gastrointestinal cancer because of its high sensitivity and specificity.
Journal Cover

September 1997
Volume 4 Issue 5

Print ISSN: 1021-335X
Online ISSN:1791-2431

2014 Impact Factor: 2.301
Ranked #31/211 Oncology
(total number of cites)

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APA
Fujimura, T., Yonemura, Y., Ninomiya, I., Miwa, K., Miyazaki, I., Endo, Y. ... Sasaki, T. (1997). Early detection of peritoneal dissemination of gastrointestinal cancers by reverse-transcriptase polymerase chain reaction. Oncology Reports, 4, 1015-1019. http://dx.doi.org/10.3892/or.4.5.1015
MLA
Fujimura, T., Yonemura, Y., Ninomiya, I., Miwa, K., Miyazaki, I., Endo, Y., Ishikawa, N., Obata, T., Tanaka, M., Sasaki, T."Early detection of peritoneal dissemination of gastrointestinal cancers by reverse-transcriptase polymerase chain reaction". Oncology Reports 4.5 (1997): 1015-1019.
Chicago
Fujimura, T., Yonemura, Y., Ninomiya, I., Miwa, K., Miyazaki, I., Endo, Y., Ishikawa, N., Obata, T., Tanaka, M., Sasaki, T."Early detection of peritoneal dissemination of gastrointestinal cancers by reverse-transcriptase polymerase chain reaction". Oncology Reports 4, no. 5 (1997): 1015-1019. http://dx.doi.org/10.3892/or.4.5.1015