IL-12 or IL-15, unlike IL-2, does not interact with histamine in augmenting cytotoxicity of splenocytes against melanoma cells and YAC-1 cells

  • Authors: Katarzyna Kozar, Rafal Kaminski, Adam Giermasz, Grzegorz Basak, Radoslaw Zagozdzon, Jolanta Rybczynska, Maria Wasik, Witold Lasek, Marek Jakobisiak, Jakub Golab
  • View Affiliations

  • Published online on: Friday, March 1, 2002
  • Pages: 427-431
  • DOI: 10.3892/or.9.2.427

Abstract

It has been suggested that histamine by its ability to downregulate the production of macrophage-derived reactive oxygen species might effectively potentiate the cytotoxic activity of cytokine-stimulated NK cells. Histamine thus reverses negative regulation of NK cells treated with IL-2 and IFN-α in the presence of macrophages. We confirm that histamine potently enhances cytotoxic activity of IL-2-stimulated NK cell-enriched splenocytes admixed with macrophages against B16F10 melanoma cells and YAC-1 cells. This stimulation results in production of high amounts of INF-γ and TNF-α. Interestingly, IL-15 by itself promotes production of reactive oxygen species. Although histamine decreased reactive oxygen species production from the cultures of IL-15-stimulated NK cell-enriched splenocytes admixed with macrophages, it did not potentiate the cytotoxicity of IL-15. Further, we demonstrate that histamine-mediated potentiation of cytotoxicity is not applicable to IL-12, another potent activator of NK cell activity.
Journal Cover

March 2002
Volume 9 Issue 2

Print ISSN: 1021-335X
Online ISSN:1791-2431

2013 Impact Factor: 2.191
Ranked #33/202 Oncology
(total number of cites)

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
APA
Kozar, K., Kaminski, R., Giermasz, A., Basak, G., Zagozdzon, R., Rybczynska, J., Wasik, M., Lasek, W., Jakobisiak, M., & Golab, J. (2002). IL-12 or IL-15, unlike IL-2, does not interact with histamine in augmenting cytotoxicity of splenocytes against melanoma cells and YAC-1 cells. Oncology Reports, 9(2), 427-431.
MLA
Kozar, Kaminski, Giermasz, Basak, Zagozdzon, Rybczynska, Wasik, Lasek, Jakobisiak, and Jakub Golab. "IL-12 or IL-15, unlike IL-2, does not interact with histamine in augmenting cytotoxicity of splenocytes against melanoma cells and YAC-1 cells." Oncology Reports Oncology Reports 9.2 (2002): 427-431.
Chicago
Kozar, Kaminski, Giermasz, Basak, Zagozdzon, Rybczynska, Wasik, Lasek, Jakobisiak, and Jakub Golab. "IL-12 or IL-15, unlike IL-2, does not interact with histamine in augmenting cytotoxicity of splenocytes against melanoma cells and YAC-1 cells." Oncology Reports Oncology Reports 9 no. 2 (2002): 427-431.