IL-12 or IL-15, unlike IL-2, does not interact with histamine in augmenting cytotoxicity of splenocytes against melanoma cells and YAC-1 cells

  • Authors:
    • Katarzyna Kozar
    • Rafal Kaminski
    • Adam Giermasz
    • Grzegorz Basak
    • Radoslaw Zagozdzon
    • Jolanta Rybczynska
    • Maria Wasik
    • Witold Lasek
    • Marek Jakobisiak
    • Jakub Golab
  • Corresponding author:
  • View Affiliations

  • Published online on: Friday, March 1, 2002
  • Pages: 427-431 DOI: 10.3892/or.9.2.427

Abstract

It has been suggested that histamine by its ability to downregulate the production of macrophage-derived reactive oxygen species might effectively potentiate the cytotoxic activity of cytokine-stimulated NK cells. Histamine thus reverses negative regulation of NK cells treated with IL-2 and IFN-α in the presence of macrophages. We confirm that histamine potently enhances cytotoxic activity of IL-2-stimulated NK cell-enriched splenocytes admixed with macrophages against B16F10 melanoma cells and YAC-1 cells. This stimulation results in production of high amounts of INF-γ and TNF-α. Interestingly, IL-15 by itself promotes production of reactive oxygen species. Although histamine decreased reactive oxygen species production from the cultures of IL-15-stimulated NK cell-enriched splenocytes admixed with macrophages, it did not potentiate the cytotoxicity of IL-15. Further, we demonstrate that histamine-mediated potentiation of cytotoxicity is not applicable to IL-12, another potent activator of NK cell activity.
Journal Cover

March 2002
Volume 9 Issue 2

Print ISSN: 1021-335X
Online ISSN:1791-2431

2013 Impact Factor: 2.191
2014 I.F. (Expected) ≥ 2.527 Ranked #33/202 Oncology
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APA
Kozar, K., Kaminski, R., Giermasz, A., Basak, G., Zagozdzon, R., Rybczynska, J. ... Golab, J. (2002). IL-12 or IL-15, unlike IL-2, does not interact with histamine in augmenting cytotoxicity of splenocytes against melanoma cells and YAC-1 cells. Oncology Reports, 9, 427-431. http://dx.doi.org/10.3892/or.9.2.427
MLA
Kozar, K., Kaminski, R., Giermasz, A., Basak, G., Zagozdzon, R., Rybczynska, J., Wasik, M., Lasek, W., Jakobisiak, M., Golab, J."IL-12 or IL-15, unlike IL-2, does not interact with histamine in augmenting cytotoxicity of splenocytes against melanoma cells and YAC-1 cells". Oncology Reports 9.2 (2002): 427-431.
Chicago
Kozar, K., Kaminski, R., Giermasz, A., Basak, G., Zagozdzon, R., Rybczynska, J., Wasik, M., Lasek, W., Jakobisiak, M., Golab, J."IL-12 or IL-15, unlike IL-2, does not interact with histamine in augmenting cytotoxicity of splenocytes against melanoma cells and YAC-1 cells". Oncology Reports 9, no. 2 (2002): 427-431. http://dx.doi.org/10.3892/or.9.2.427