Rosmarinic acid and arbutin suppress osteoclast differentiation by inhibiting superoxide and NFATc1 downregulation in RAW 264.7 cells

  • Authors:
    • Akina Omori
    • Yoshitaka Yoshimura
    • Yoshiaki Deyama
    • Kuniaki Suzuki
  • View Affiliations

  • Published online on: April 17, 2015     https://doi.org/10.3892/br.2015.452
  • Pages: 483-490
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Abstract

The present study investigated the effect of the natural polyphenols, rosmarinic acid and arbutin, on osteoclast differentiation in RAW 264.7 cells. Rosmarinic acid and arbutin suppressed osteoclast differentiation and had no cytotoxic effect on osteoclast precursor cells. Rosmarinic acid and arbutin inhibited superoxide production in a dose‑dependent manner. mRNA expression of the master regulator of osteoclastogenesis, nuclear factor of activated T cells cytoplasmic 1 (NFATc1) and the osteoclast marker genes, matrix metalloproteinase‑9, tartrate‑resistant acid phosphatase and cathepsin‑K, decreased following treatments with rosmarinic acid and arbutin. Furthermore, resorption activity decreased with the number of osteoclasts. These results suggest that rosmarinic acid and arbutin may be useful for the prevention and treatment of bone diseases, such as osteoporosis, through mechanisms involving inhibition of superoxide and downregulation of NFATc1.
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July-2015
Volume 3 Issue 4

Print ISSN: 2049-9434
Online ISSN:2049-9442

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Spandidos Publications style
Omori A, Yoshimura Y, Deyama Y and Suzuki K: Rosmarinic acid and arbutin suppress osteoclast differentiation by inhibiting superoxide and NFATc1 downregulation in RAW 264.7 cells. Biomed Rep 3: 483-490, 2015
APA
Omori, A., Yoshimura, Y., Deyama, Y., & Suzuki, K. (2015). Rosmarinic acid and arbutin suppress osteoclast differentiation by inhibiting superoxide and NFATc1 downregulation in RAW 264.7 cells. Biomedical Reports, 3, 483-490. https://doi.org/10.3892/br.2015.452
MLA
Omori, A., Yoshimura, Y., Deyama, Y., Suzuki, K."Rosmarinic acid and arbutin suppress osteoclast differentiation by inhibiting superoxide and NFATc1 downregulation in RAW 264.7 cells". Biomedical Reports 3.4 (2015): 483-490.
Chicago
Omori, A., Yoshimura, Y., Deyama, Y., Suzuki, K."Rosmarinic acid and arbutin suppress osteoclast differentiation by inhibiting superoxide and NFATc1 downregulation in RAW 264.7 cells". Biomedical Reports 3, no. 4 (2015): 483-490. https://doi.org/10.3892/br.2015.452