Effect of advanced glycation end products, extracellular matrix metalloproteinase inducer and matrix metalloproteinases on type‑I collagen metabolism
- Authors:
- Wang Li
- Wang Ling
- Xiaomei Teng
- Cuixia Quan
- Shengnan Cai
- Shuqun Hu
View Affiliations
Affiliations: Department of Clinical Laboratory, Xuzhou First People's Hospital, Xuzhou, Jiangsu 221002, P.R. China, Teaching and Research Section of Biochemistry, Xuzhou Medical College, Xuzhou, Jiangsu 221004, P.R. China
- Published online on: March 28, 2016 https://doi.org/10.3892/br.2016.641
-
Pages:
691-693
-
Copyright: © Li
et al. This is an open access article distributed under the
terms of Creative
Commons Attribution License.
Metrics: Total
Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
This article is mentioned in:
Abstract
The aim of the study was to examine the association among advanced glycation end products (AGEs), extracellular matrix metalloproteinase inducer (EMMPRIN) and matrix metalloproteinase (MMPs), and investigate whether AGEs affect type I collagen (COL‑I) through EMMPRIN or MMPs. A co‑culture system with the osteoblast‑like cells (MC3T3E1) and mouse RAW264.7 cells was employed to examine the effects of AGE‑bovine serum albumin (BSA) (50 mg/l), EMMPRIN antibody (5 mg/l) and AGE‑BSA+EMMPRIN antibody separately on COL‑I expression for 24 h. Culture media were analyzed for the content of COL‑I by ELISA. The effect of different concentrations of AGE‑BSA (0, 50, 100, 200 and 400 mg/l) for 24 h was assessed on COL‑I levels. Finally, semiquantitative RT‑PCR was used to detect the osteoblast COL‑I mRNA expression and MMP‑2 and MMP‑9's PMAO were also measured in the culture medium. COL‑I content in the culture medium decreased significantly following treatment with AGE‑BSA (P<0.05). EMMPRIN antibody increased COL‑I content (P<0.05). EMMPRIN antibody+AGE‑BSA increased COL‑I significantly (P<0.05). Different concentrations of AGE‑BSA increased COL‑I mRNA expression significantly compared with the control group (P<0.05), and were enhanced with increasing AGE‑BSA concentration (P<0.05). Also MMP‑2 and MMP‑9 secretion increased significantly (P<0.05), with the increasing AGE‑BSA concentration. In conclusion, an increase in AGE levels in vitro stimulates the secretion of EMMPRIN/MMPs, promotes the degradation of COL‑I and reduces bone strength.
View References
1
|
Liu J, Xu Z, Wang J, et al: The changes of
metabolism of collagen type i in post-menopausal osteoporosis. Acta
Anat Sin. 33:166–169. 2002.
|
2
|
Everts V, Korper W, Docherty AJ and
Beertsen W: Matrix metalloproteinase inhibitors block osteoclastic
resorption of calvarial bone but not the resorption of long bone.
Ann N Y Acad Sci. 878:603–606. 1999. View Article : Google Scholar : PubMed/NCBI
|
3
|
Ji JD, Woo JH, Choi SJ, Lee YH and Song
GG: Advanced glycation end-products (AGEs): A novel therapeutic
target for osteoporosis in patients with rheumatoid arthritis. Med
Hypotheses. 73:201–202. 2009. View Article : Google Scholar : PubMed/NCBI
|
4
|
Dong XN, Qin A, Xu J and Wang X: In situ
accumulation of advanced glycation endproducts (AGEs) in bone
matrix and its correlation with osteoclastic bone resorption. Bone.
49:174–183. 2011. View Article : Google Scholar : PubMed/NCBI
|
5
|
Hein G, Wiegand R, Lehmann G, Stein G and
Franke S: Advanced glycation end-products pentosidine and
Nε-carboxymethyllysine are elevated in serum of patients
with osteoporosis. Rheumatology (Oxford). 42:1242–1246. 2003.
View Article : Google Scholar : PubMed/NCBI
|
6
|
Xie B, Chen Y and Liu H: The role of
collagen in osteoporosis. Chin J Gerontol. 29:2057–2059. 2009.
|
7
|
Yamamoto T and Ozono K: Role of advanced
glycation endproducts in adynamic bone disease. Clin Calcium.
11:1044–1047. 2001.(In Japanese). PubMed/NCBI
|
8
|
Dai R, Jin H and Sun Z: Effect of advanced
glycation end products on the gene expression of EMMPRIN in
cultured mouse embryo/fetus calvaria fibroblasts. Jiangsu Med J.
33:913–915. 2007.(In Chinese).
|
9
|
Qin J, Wang Z and Bi S: Relationship
between serum matrix metalloproteinase-2, serum matrix
metalloproteinase-9,bone biochemical markers and bone mineral
density in postmenopausal women. Chin J Osteoporos. 15:292–295.
2009.
|
10
|
Zhou J, Zhu P, Jiang JL, Zhang Q, Wu ZB,
Yao XY, Tang H, Lu N, Yang Y and Chen ZN: Involvement of CD147 in
overexpression of MMP-2 and MMP-9 and enhancement of invasive
potential of PMA-differentiated THP-1. BMC Cell Biol. 6:252005.
View Article : Google Scholar : PubMed/NCBI
|
11
|
Xie B, Chen Y and Liu H: Effect of bone
collagen in osteoporosis. Chin J Gerontol. 29:2057–2059. 2009.
|