Open Access

Differences in the reaction of hyperlipidemia on different endothelial progenitor cells based on sex

  • Authors:
    • Xiaoqing Zhuo
    • Haoran Bu
    • Ke Hu
    • Zhihua Si
    • Liming Chen
    • Yong Chen
    • Le Yang
    • Yufan Jiang
    • Yixin Xu
    • Peng Zhao
    • Xiaochun Ma
    • Shufei Tao
    • Qingyi Zhu
    • Lianqun Cui
    • Haihui Sun
    • Yuqi Cui
  • View Affiliations

  • Published online on: June 7, 2021     https://doi.org/10.3892/br.2021.1440
  • Article Number: 64
  • Copyright: © Zhuo et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The sex of a patient can affect the outcomes of several cardiovascular diseases, and men generally tend to experience earlier episodes of cardiovascular diseases compared with women. The progression of atherosclerosis during hyperlipidemia can be induced by reactive oxygen species (ROS) and oxidized‑low‑density lipoprotein (ox‑LDL). By contrast, bone marrow (BM)‑derived endothelial progenitor cells (EPCs) have been reported to serve a protective role against atherosclerosis. The aim of the present was to compare the effects of sex under conditions of hyperlipidemia on different populations of EPCs, and to identify the potential underlying mechanisms. EPC numbers and ROS levels in the blood and BM were measured using fluorescence activated cell sorting in male and female LDL receptor knock‑out C57BL/6 mice maintained on a high‑fat diet for 6 months, and in male and female wild type C57BL/6 mice following ox‑LDL injection for 3 days. Female hyperlipidemic mice exhibited lower levels of plasma lipids, atherosclerotic plaque formation, intracellular EPC ROS formation and inflammatory cytokine levels. Furthermore, BM CD34+/ fetal liver kinase‑1 (Flk‑1+), CD34+/CD133+ and stem cell antigen‑1+/Flk‑1+, as well as all circulating EPCs, were maintained at higher levels in female hyperlipidemic mice. In addition, similar changes with regards to BM CD34+/Flk‑1+, CD34+/CD133+, c‑Kit+/CD31+ and circulating CD34+/Flk1+ and CD34+/CD133+ EPCs were observed in female mice following ox‑LDL treatment. These sustained higher levels of BM and circulating EPCs in female mice with hyperlipidemia may be associated with reduced levels of ox‑LDL as a result of reduced intracellular ROS formation in EPCs and decreased inflammatory cytokine production.
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August-2021
Volume 15 Issue 2

Print ISSN: 2049-9434
Online ISSN:2049-9442

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Copy and paste a formatted citation
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Spandidos Publications style
Zhuo X, Bu H, Hu K, Si Z, Chen L, Chen Y, Yang L, Jiang Y, Xu Y, Zhao P, Zhao P, et al: Differences in the reaction of hyperlipidemia on different endothelial progenitor cells based on sex. Biomed Rep 15: 64, 2021
APA
Zhuo, X., Bu, H., Hu, K., Si, Z., Chen, L., Chen, Y. ... Cui, Y. (2021). Differences in the reaction of hyperlipidemia on different endothelial progenitor cells based on sex. Biomedical Reports, 15, 64. https://doi.org/10.3892/br.2021.1440
MLA
Zhuo, X., Bu, H., Hu, K., Si, Z., Chen, L., Chen, Y., Yang, L., Jiang, Y., Xu, Y., Zhao, P., Ma, X., Tao, S., Zhu, Q., Cui, L., Sun, H., Cui, Y."Differences in the reaction of hyperlipidemia on different endothelial progenitor cells based on sex". Biomedical Reports 15.2 (2021): 64.
Chicago
Zhuo, X., Bu, H., Hu, K., Si, Z., Chen, L., Chen, Y., Yang, L., Jiang, Y., Xu, Y., Zhao, P., Ma, X., Tao, S., Zhu, Q., Cui, L., Sun, H., Cui, Y."Differences in the reaction of hyperlipidemia on different endothelial progenitor cells based on sex". Biomedical Reports 15, no. 2 (2021): 64. https://doi.org/10.3892/br.2021.1440