Expression of nerve growth factor in the prostate of male rats in response to chronic stress and sympathetic denervation

Huang,Shengliang; Zhang,Xuebei; Xu,Liuyu; Li,Qing; Zhao,Qingli

doi:10.3892/etm.2014.1856

Expression of nerve growth factor in the prostate of male rats in response to chronic stress and sympathetic denervation

Authors:
- Shengliang Huang
- Xuebei Zhang
- Liuyu Xu
- Qing Li
- Qingli Zhao
View Affiliations

Affiliations: Department of Urology, Shandong Provincial Qianfoshan Hospital, Shandong University, Jinan, Shandong 250014, P.R. China, Department of Emergency, Qilu Hospital of Shandong University, Jinan, Shandong 250012, P.R. China
Published online on: July 21, 2014 https://doi.org/10.3892/etm.2014.1856
Pages: 1237-1240

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

Nerve growth factor (NGF) has been found in the normal prostate of the Wistar rat and is regarded as an important prostatic mitogen. We have previously shown that chronic stress induced epithelial hyperplasia while sympathetic denervation caused atrophy in the male Wistar rat prostate. NGF may have been a contributing mechanism to the hyperplasia and atrophy response that was observed. The aim of the present study was to investigate the expression of NGF in the prostate of the male rat in response to chronic stress and denervation. Two weeks of restraint water‑immersion stress were used to induce a chronic stress model in Wistar rats. Denervation of the peripheral sympathetic nerve was induced by 6‑hydroxydopamine. The expression levels of NGF in the dissected prostate lobes were examined by immunohistochemistry. After 14 days of stress, proliferation of the epithelium in the ventral lobes was observed, whereas the dorsolateral lobes were almost unaffected. NGF immunoreactive protein was localized to the columnar secretory epithelium lines of the prostate tissue. Stress and denervation led to an increase in NGF expression in the ventral lobes. In conclusion, NGF was involved in the hyperplasia and atrophy in the prostate of the male rat in response to chronic stress and sympathetic denervation, and thus may be a contributing factor in the pathophysiology of the prostate.

View Figures

View References

1	Snider WD: Functions of the neurotrophins during nervous system development: what the knockouts are teaching us. Cell. 77:627–638. 1994. View Article : Google Scholar : PubMed/NCBI
2	Paul AB, Grant ES and Habib FK: The expression and localisation of beta-nerve growth factor (beta-NGF) in benign and malignant human prostate tissue: relationship to neuroendocrine differentiation. Br J Cancer. 74:1990–1996. 1996. View Article : Google Scholar : PubMed/NCBI
3	Papatsoris AG, Liolitsa D and Deliveliotis C: Manipulation of the nerve growth factor network in prostate cancer. Expert Opin Investig Drugs. 16:303–309. 2007. View Article : Google Scholar : PubMed/NCBI
4	Arrighi N, Bodei S, Zani D, et al: Nerve growth factor signaling in prostate health and disease. Growth Factors. 28:191–201. 2010. View Article : Google Scholar : PubMed/NCBI
5	McVary KT, McKenna KE and Lee C: Prostate innervation. Prostate Suppl. 8:2–13. 1998. View Article : Google Scholar
6	Li C, Watanabe G, Weng Q, et al: Expression of nerve growth factor (NGF), and its receptors TrkA and p75 in the reproductive organs of the adult male rats. Zoolog Sci. 22:933–937. 2005. View Article : Google Scholar : PubMed/NCBI
7	Huang S, Fang X, Meng Y, et al: Sympathetic nervous system overactivity in the Wistar rat with proliferative lesions of ventral prostate induced by chronic stress. Urol Int. 83:230–235. 2009. View Article : Google Scholar : PubMed/NCBI
8	Vaalasti A, Alho AM, Tainio H and Hervonen A: The effect of sympathetic denervation with 6-hydroxydopamine on the ventral prostate of the rat. Acta Histochem. 79:49–54. 1986. View Article : Google Scholar : PubMed/NCBI
9	Karp JD, Smith J and Hawk K: Restraint stress augments antibody production in cyclophosphamide-treated mice. Physiol Behav. 70:271–278. 2000. View Article : Google Scholar : PubMed/NCBI
10	Murphy RA, Watson AY and Rhodes JA: Biological sources of nerve growth factor. Appl Neurophysiol. 47:33–42. 1984.PubMed/NCBI
11	Djakiew D, Delsite R, Pflug B, et al: Regulation of growth by a nerve growth factor-like protein which modulates paracrine interactions between a neoplastic epithelial cell line and stromal cells of the human prostate. Cancer Res. 51:3304–3310. 1991.
12	Graham CW, Lynch JH and Djakiew D: Distribution of nerve growth factor-like protein and nerve growth factor receptor in human benign prostatic hyperplasia and prostatic adenocarcinoma. J Urol. 147:1444–1447. 1992.PubMed/NCBI
13	Liu DT, Reid MT, Bridges DC and Rush RA: Denervation, but not decentralization, reduces nerve growth factor content of the mesenteric artery. J Neurochem. 66:2295–2299. 1996.PubMed/NCBI
14	Goda M, Atagi S, Amitani K, et al: Nerve growth factor suppresses prostate tumor growth. J Pharmacol Sci. 112:463–466. 2010. View Article : Google Scholar : PubMed/NCBI
15	Golomb E, Rosenzweig N, Eilam R and Abramovici A: Spontaneous hyperplasia of the ventral lobe of the prostate in aging genetically hypertensive rats. J Androl. 21:58–64. 2000.PubMed/NCBI
16	Clemow DB, Steers WD, McCarty R and Tuttle JB: Altered regulation of bladder nerve growth factor and neurally mediated hyperactive voiding. Am J Physiol. 275:R1279–R1286. 1998.PubMed/NCBI
17	Kim JC, Park EY, Hong SH, et al: Changes of urinary nerve growth factor and prostaglandins in male patients with overactive bladder symptom. Int J Urol. 12:875–880. 2005. View Article : Google Scholar : PubMed/NCBI
18	Djakiew D: Dysregulated expression of growth factors and their receptors in the development of prostate cancer. Prostate. 42:150–160. 2000. View Article : Google Scholar : PubMed/NCBI
19	Dalal R and Djakiew D: Molecular characterization of neurotrophin expression and the corresponding tropomyosin receptor kinases (trks) in epithelial and stromal cells of the human prostate. Mol Cell Endocrinol. 134:15–22. 1997. View Article : Google Scholar