Mitochondrial separation protein inhibitor inhibits cell apoptosis in rat lungs during intermittent hypoxia

  • Authors:
    • Dan Zhao
    • Chen‑Yi Yin
    • Xian‑Wei Ye
    • Zi‑Fen Wan
    • De‑Gang Zhao
    • Xiang‑Yan Zhang
  • View Affiliations

  • Published online on: January 25, 2019     https://doi.org/10.3892/etm.2019.7201
  • Pages: 2349-2358
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Abstract

Obstructive sleep apnoea (OSA) is a very common sleep and breathing disorder that occurs in worldwide. It is important to develop a more effective treatment for OSA to overcome lung cell apoptosis during intermittent hypoxia (IH). A mitochondrial separation protein inhibitor (Mdivi‑1) has been demonstrated to be a powerful tool for inhibiting apoptosis. In the present study, the protective effect and possible mechanism of apoptosis in lung cells during IH was investigated using in vivo and in vitro experiments. Following IH exposure for 4 weeks, the lung tissues of Sprague Dawley rats exhibited interstitial lesions, while Mdivi‑1 reduced these pulmonary interstitial lesions. B‑cell lymphoma (Bcl)‑2 mRNA and protein expression levels were decreased however caspase‑3, caspase‑9 and dynamin‑related protein 1 (Drp‑1) mRNA and protein expression levels were increased. Following Mdivi‑1 intervention, Bcl‑2 mRNA and protein expression levels were increased while caspase‑3, caspase‑9 and Drp‑1 mRNA and protein expression levels were decreased (P<0.05). After exposure to IH for 12 h, the apoptosis rate of WTRL1 cells in rats increased gradually with the IH time (P<0.05). Bcl‑2 mRNA and protein expression levels were decreased, whereas caspase‑3, caspase‑9, cytochrome C (Cyt‑C) and Drp‑1 mRNA levels were increased, and caspase‑3, caspase‑9 and Drp‑1 protein expression levels were increased. After Mdivi‑1 intervention, Bcl‑2 mRNA and protein expression levels were increased but caspase‑3, caspase‑9, Cyt‑C and Drp‑1 mRNA levels were decreased along with caspase‑9, Cyt‑C and Drp‑1 protein expression levels which were decreased (P<0.05). The results of the present study suggest that Mdivi‑1 may be a potential agent for treating OSA because it inhibits the mitochondrial pathway and reduces apoptosis.
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March-2019
Volume 17 Issue 3

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Zhao D, Yin CY, Ye XW, Wan ZF, Zhao DG and Zhang XY: Mitochondrial separation protein inhibitor inhibits cell apoptosis in rat lungs during intermittent hypoxia . Exp Ther Med 17: 2349-2358, 2019.
APA
Zhao, D., Yin, C., Ye, X., Wan, Z., Zhao, D., & Zhang, X. (2019). Mitochondrial separation protein inhibitor inhibits cell apoptosis in rat lungs during intermittent hypoxia . Experimental and Therapeutic Medicine, 17, 2349-2358. https://doi.org/10.3892/etm.2019.7201
MLA
Zhao, D., Yin, C., Ye, X., Wan, Z., Zhao, D., Zhang, X."Mitochondrial separation protein inhibitor inhibits cell apoptosis in rat lungs during intermittent hypoxia ". Experimental and Therapeutic Medicine 17.3 (2019): 2349-2358.
Chicago
Zhao, D., Yin, C., Ye, X., Wan, Z., Zhao, D., Zhang, X."Mitochondrial separation protein inhibitor inhibits cell apoptosis in rat lungs during intermittent hypoxia ". Experimental and Therapeutic Medicine 17, no. 3 (2019): 2349-2358. https://doi.org/10.3892/etm.2019.7201