Chrysoeriol ameliorates COX-2 expression through NF-κB, AP-1 and MAPK regulation via the TLR4/MyD88 signaling pathway in LPS-stimulated murine macrophages

  • Authors:
    • Hyun-Seo Yoon
    • Chung Mu Park
  • View Affiliations

  • Published online on: May 3, 2021     https://doi.org/10.3892/etm.2021.10150
  • Article Number: 718
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Abstract

Chrysoeriol is a flavonoid that has diverse biological properties, including antioxidation, anti‑inflammation, chemoprevention and immunomodulation. Despite its reported anti‑inflammatory activity, the exact underlying molecular mechanism has not yet been elucidated. In the current study, the anti‑inflammatory mechanism of chrysoeriol involving lipopolysaccharide (LPS)‑induced cyclooxygenase‑2 (COX-2) and its upstream signaling molecules was investigated in RAW 264.7 cells. The mechanism was evaluated via ELISA and western blotting assays. Chrysoeriol significantly inhibited LPS‑induced prostaglandin E2 (PGE2) production and COX‑2 expression without cytotoxicity. Activated transcription factors that further induced the inflammation response, including nuclear factor (NF)‑κB and activator protein‑1 (AP‑1), were significantly attenuated by chrysoeriol treatment. Furthermore, LPS‑induced phosphorylation levels of phosphoinositide‑3‑kinase (PI3K)/Akt and mitogen‑activated protein kinase (MAPK) were abolished by chrysoeriol treatment, which was confirmed by selective inhibitors. Additionally, chrysoeriol significantly inhibited the LPS‑induced activation of adaptor molecules in RAW 264.7 cells, including toll‑like receptor 4 (TLR4) and myeloid differentiation primary response 88. Therefore, the results suggested that chrysoeriol ameliorates TLR4‑mediated inflammatory responses by inhibiting NF‑κB and AP‑1 activation as well as suppressing PI3K/Akt and MAPK phosphorylation in LPS‑stimulated RAW 264.7 cells.
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July-2021
Volume 22 Issue 1

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Yoon H and Park CM: Chrysoeriol ameliorates COX-2 expression through NF-κB, AP-1 and MAPK regulation via the TLR4/MyD88 signaling pathway in LPS-stimulated murine macrophages. Exp Ther Med 22: 718, 2021.
APA
Yoon, H., & Park, C.M. (2021). Chrysoeriol ameliorates COX-2 expression through NF-κB, AP-1 and MAPK regulation via the TLR4/MyD88 signaling pathway in LPS-stimulated murine macrophages. Experimental and Therapeutic Medicine, 22, 718. https://doi.org/10.3892/etm.2021.10150
MLA
Yoon, H., Park, C. M."Chrysoeriol ameliorates COX-2 expression through NF-κB, AP-1 and MAPK regulation via the TLR4/MyD88 signaling pathway in LPS-stimulated murine macrophages". Experimental and Therapeutic Medicine 22.1 (2021): 718.
Chicago
Yoon, H., Park, C. M."Chrysoeriol ameliorates COX-2 expression through NF-κB, AP-1 and MAPK regulation via the TLR4/MyD88 signaling pathway in LPS-stimulated murine macrophages". Experimental and Therapeutic Medicine 22, no. 1 (2021): 718. https://doi.org/10.3892/etm.2021.10150