Aberrant expression of miR‑483‑5p in patients with asymptomatic carotid artery stenosis and its predictive value for cerebrovascular event occurrence

Li,Ran; Jiang,Li; Wang,Xiaoling

doi:10.3892/etm.2021.10536

Aberrant expression of miR‑483‑5p in patients with asymptomatic carotid artery stenosis and its predictive value for cerebrovascular event occurrence

Authors:
- Ran Li
- Li Jiang
- Xiaoling Wang
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Affiliations: The Fourth Department of Encephalopathy, Weifang Hospital of Traditional Chinese Medicine, Weifang, Shandong 261041, P.R. China, Brain Center, Sunshine Union Hospital, Weifang, Shandong 261000, P.R. China, Department of Neurology, Liaocheng People's Hospital, Liaocheng, Shandong 252000, P.R. China
Published online on: August 2, 2021 https://doi.org/10.3892/etm.2021.10536
Article Number: 1101
Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

MicroRNAs (miRNAs/miRs) may be used as novel promising diagnostic and prognostic biomarkers for various diseases, including asymptomatic carotid artery stenosis (ACAS). The present study aimed to investigate the abnormal expression of microRNA‑483‑5p (miR‑483‑5p) in patients with ACAS and to evaluate its diagnostic value for ACAS screening and its predictive value for cerebrovascular events. A total of 128 patients with ACAS and 76 healthy controls were included in the present study. The expression of miR‑483‑5p in serum was measured by reverse transcription‑quantitative PCR. Receiver operating characteristic (ROC) curve analysis was used to evaluate the diagnostic value of miR‑483‑5p in patients with ACAS. Kaplan‑Meier curves were drawn and Cox regression analysis was used to determine the predictive value of miR‑483‑5p for cerebrovascular events in patients with ACAS. Serum miR‑483‑5p levels were significantly increased in patients with ACAS as compared with those in healthy controls. The expression of miR‑483‑5p was significantly associated with diabetes (P=0.011), dyslipidemia (P=0.047) and the degree of carotid stenosis (P=0.006) in patients with ACAS. In addition, the area under the ROC curve was 0.910, with a sensitivity of 80.5% and a specificity of 89.5% at the cutoff value of 0.705, indicating that serum miR‑483‑5p expression has a certain diagnostic value in patients with ACAS. Furthermore, the patients with high miR‑483‑5p expression had a higher proportion of cerebrovascular events than patients with low miR‑483‑5p levels (log‑rank P=0.011) and miR‑483‑5p was an independent prognostic marker for predicting the occurrence of cerebrovascular events in patients with ACAS. The results indicated that miR‑483‑5p expression is significantly increased in patients with ACAS and that abnormal miR‑483‑5p expression may be a candidate biomarker for ACAS diagnosis and the prediction of cerebrovascular event occurrence.

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