Open Access

MicroRNA-98-5p inhibits human mesangial cell proliferation and TNF-α and IL‑6 secretion by targeting BTB and CNC homology 1

  • Authors:
    • Yong Du
    • Xiaoqiang Shi
    • Jing Li
    • Yanhui Jia
  • View Affiliations

  • Published online on: October 12, 2021     https://doi.org/10.3892/etm.2021.10871
  • Article Number: 1436
  • Copyright: © Du et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

MicroRNA (miR)‑98‑5p has been reported to be involved in the development of lupus nephritis (LN); however, its specific role in LN remains unclear. The present study aimed to investigate the effect of miR‑98‑5p on human mesangial cell proliferation and the secretion of TNF‑α and IL‑6. Reverse transcription‑quantitative PCR (RT‑qPCR) and western blotting were used to analyze the level of gene and protein expression, respectively. Cellular proliferation was assessed using a Cell Counting Kit‑8 (CCK‑8) assay. ELISA was used to detect the secretion of TNF‑α and IL‑6 by human mesangial cells. RT‑qPCR analysis revealed that miR‑98‑5p expression was downregulated in LN renal tissues compared with control renal tissues. Overexpression of miR‑98‑5p inhibited human mesangial cell proliferation and the secretion of TNF‑α and IL‑6, whereas miR‑98‑5p‑knockdown demonstrated the opposite effect. Dual luciferase reporter assays demonstrated that miR‑98‑5p directly targeted BTB and CNC homology 1 (BACH1). BACH1‑overexpression promoted human mesangial cell proliferation and the secretion of TNF‑α and IL‑6, whereas BACH1‑knockdown demonstrated the opposite effect. Notably, co‑transfection with miR‑98‑5p mimic inhibited BACH1‑overexpression induced human mesangial cell proliferation and the secretion of TNF‑α and IL‑6. The results of the present study indicated that miR‑98‑5p inhibited human mesangial cell proliferation and the secretion of TNF‑α and IL‑6 by targeting BACH1. Therefore, miR‑98‑5p and BACH1 may represent potential therapeutic targets for LN.
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December-2021
Volume 22 Issue 6

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Du Y, Shi X, Li J and Jia Y: MicroRNA-98-5p inhibits human mesangial cell proliferation and TNF-α and IL‑6 secretion by targeting BTB and CNC homology 1. Exp Ther Med 22: 1436, 2021.
APA
Du, Y., Shi, X., Li, J., & Jia, Y. (2021). MicroRNA-98-5p inhibits human mesangial cell proliferation and TNF-α and IL‑6 secretion by targeting BTB and CNC homology 1. Experimental and Therapeutic Medicine, 22, 1436. https://doi.org/10.3892/etm.2021.10871
MLA
Du, Y., Shi, X., Li, J., Jia, Y."MicroRNA-98-5p inhibits human mesangial cell proliferation and TNF-α and IL‑6 secretion by targeting BTB and CNC homology 1". Experimental and Therapeutic Medicine 22.6 (2021): 1436.
Chicago
Du, Y., Shi, X., Li, J., Jia, Y."MicroRNA-98-5p inhibits human mesangial cell proliferation and TNF-α and IL‑6 secretion by targeting BTB and CNC homology 1". Experimental and Therapeutic Medicine 22, no. 6 (2021): 1436. https://doi.org/10.3892/etm.2021.10871