Effects of mulberry ethanol extracts on hydrogen peroxide-induced oxidative stress in pancreatic β-cells

  • Authors:
    • Young Rae Kim
    • Jong Seok Lee
    • Ki Rim Lee
    • Young Eon Kim
    • Nam In Baek
    • Eock Kee Hong
  • View Affiliations

  • Published online on: October 23, 2013     https://doi.org/10.3892/ijmm.2013.1534
  • Pages: 128-134
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Abstract

Reactive oxygen species (ROS) are key mediators of mammalian cellular damage and are associated with diseases such as aging, arteriosclerosis, inflammation, rheumatoid arthritis and diabetes. Type 1 diabetes develops upon the destruction of pancreatic β-cells, which is partly due to ROS activity. In this study, we investigated the cytoprotective and anti-oxidative effects of fractionated mulberry extracts in mouse insulin-producing pancreatic β-cells (MIN6N cells). Treatment with hydrogen peroxide (H2O2) induced significant cell death and increased intracellular ROS levels, lipid peroxidation and DNA fragmentation in the MIN6N cells. Fractionated mulberry extracts significantly reduced the H2O2-dependent production of intracellular ROS, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals and lipid peroxidation. In addition, mulberry extracts inhibited DNA fragmentation induced by H2O2. Thus, the antioxidant properties of mulberry extracts in pancreatic β-cells may be exploited for the prevention or treatment of type 1 diabetes.
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2014-January
Volume 33 Issue 1

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Kim YR, Lee JS, Lee KR, Kim YE, Baek NI and Hong EK: Effects of mulberry ethanol extracts on hydrogen peroxide-induced oxidative stress in pancreatic β-cells. Int J Mol Med 33: 128-134, 2014
APA
Kim, Y.R., Lee, J.S., Lee, K.R., Kim, Y.E., Baek, N.I., & Hong, E.K. (2014). Effects of mulberry ethanol extracts on hydrogen peroxide-induced oxidative stress in pancreatic β-cells. International Journal of Molecular Medicine, 33, 128-134. https://doi.org/10.3892/ijmm.2013.1534
MLA
Kim, Y. R., Lee, J. S., Lee, K. R., Kim, Y. E., Baek, N. I., Hong, E. K."Effects of mulberry ethanol extracts on hydrogen peroxide-induced oxidative stress in pancreatic β-cells". International Journal of Molecular Medicine 33.1 (2014): 128-134.
Chicago
Kim, Y. R., Lee, J. S., Lee, K. R., Kim, Y. E., Baek, N. I., Hong, E. K."Effects of mulberry ethanol extracts on hydrogen peroxide-induced oxidative stress in pancreatic β-cells". International Journal of Molecular Medicine 33, no. 1 (2014): 128-134. https://doi.org/10.3892/ijmm.2013.1534