Construction of a DNA vaccine based on the Mycobacterium tuberculosis Ag85A/MPT64 fusion gene and evaluation of its immunogenicity

  • Authors:
    • Hong Bao
    • Ting Yu
    • Yufen Jin
    • Chunyan Teng
    • Ximing Liu
    • Yanlei Li
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  • Published online on: September 28, 2012     https://doi.org/10.3892/mmr.2012.1109
  • Pages: 1375-1378
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Abstract

The aim of this study was to construct a DNA vaccine based on the Ag85A/MPT64 gene of Mycobacterium tuberculosis (MTB) and analyze its immunogenicity by enzyme-linked immunospot (ELISPOT) assay. The fusion gene encoding Ag85A/MPT64 was amplified by PCR from the genome of the MTB H37Rv strain and cloned into a eukaryotic expression vector followed by confirmation using restriction endonuclease digestion and DNA sequencing. The immunogenicity of the recombinant vector was tested in vivo in BALB/c mice. The serum antibody titers against Ag85A/MPT64 were detected by ELISPOT assay. The number of ELISPOT spots for the mice following immunization with Ag85A/MPT64 was significantly greater than for the negative and blank controls. A DNA vaccine based on the gene encoding the Ag85A/MPT64 fusion protein of MTB was successfully constructed and expressed. Our data may serve as a foundation for further research into the prevention and treatment of tuberculosis and carcinomas.
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December 2012
Volume 6 Issue 6

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Bao H, Yu T, Jin Y, Teng C, Liu X and Li Y: Construction of a DNA vaccine based on the Mycobacterium tuberculosis Ag85A/MPT64 fusion gene and evaluation of its immunogenicity. Mol Med Rep 6: 1375-1378, 2012
APA
Bao, H., Yu, T., Jin, Y., Teng, C., Liu, X., & Li, Y. (2012). Construction of a DNA vaccine based on the Mycobacterium tuberculosis Ag85A/MPT64 fusion gene and evaluation of its immunogenicity. Molecular Medicine Reports, 6, 1375-1378. https://doi.org/10.3892/mmr.2012.1109
MLA
Bao, H., Yu, T., Jin, Y., Teng, C., Liu, X., Li, Y."Construction of a DNA vaccine based on the Mycobacterium tuberculosis Ag85A/MPT64 fusion gene and evaluation of its immunogenicity". Molecular Medicine Reports 6.6 (2012): 1375-1378.
Chicago
Bao, H., Yu, T., Jin, Y., Teng, C., Liu, X., Li, Y."Construction of a DNA vaccine based on the Mycobacterium tuberculosis Ag85A/MPT64 fusion gene and evaluation of its immunogenicity". Molecular Medicine Reports 6, no. 6 (2012): 1375-1378. https://doi.org/10.3892/mmr.2012.1109