Regulatory effects of apoptosis-associated speck-like protein on cytokines in the P388D1 macrophage-like cell line

Ren,Shuangyi; Chen,Hailong

doi:10.3892/mmr.2012.1124

Regulatory effects of apoptosis-associated speck-like protein on cytokines in the P388D1 macrophage-like cell line

Authors:
- Shuangyi Ren
- Hailong Chen
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Affiliations: Department of Surgery, The Second Affiliated Hospital of Dalian Medical University, Dalian, Liaoning 116023, P.R. China, Department of Surgery, The First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning 116011, P.R. China
Published online on: October 12, 2012 https://doi.org/10.3892/mmr.2012.1124
Pages: 166-170

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Abstract

Apoptosis-associated speck-like protein (ASC) is an adaptor molecule of caspase-1 activation that stimulates the secretion of multiple pro-inflammatory cytokines. We investigated the regulatory effects of ASC in the P388D1 macrophage-like cell line. Data showed that ASC overexpression induced by pEGFP-ASC-C2 transfection significantly increased caspase-1 expression and interleukin (IL)-1β and IL-6 secretion, but did not affect tumor necrosis factor (TNF)-α secretion. However, siRNA against ASC significantly decreased the caspase-1 expression and IL-1β and IL-6 secretion, but did not affect TNF-α secretion in the P388D1 cells. We suggest that ASC acts as an inflammatory response-associated gene by regulating caspase-1 activation and IL-1β and IL-6 secretion, which may correlate with its biological effects.

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