Serum biomarker screening for the diagnosis of early gastric cancer using SELDI-TOF-MS

  • Authors:
    • Ping Li
    • Dianliang Zhang
    • Chunbao Guo
  • View Affiliations

  • Published online on: March 13, 2012     https://doi.org/10.3892/mmr.2012.834
  • Pages: 1531-1535
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Abstract

In this study, we performed a proteomic analysis of sera from stage I gastric cancer patients using surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF-MS) and established a diagnostic model for the early diagnosis of stage I gastric cancer. Serum samples from 169 gastric cancer patients and 83 age- and gender-matched healthy individuals were analyzed by SELDI-TOF-MS ProteinChip array technology. The SELDI-TOF-MS spectral data were analyzed using the Biomarker Wizard™ and Biomarker Patterns™ software to find differential proteins and develop a classification tree for gastric cancer. A total of 34 mass peaks were identified. Six peaks at a mass-to-charge ratio (m/z) of 2873, 3163, 4526, 5762, 6121 and 7778 were used to construct the diagnostic model. The model effectively distinguished gastric cancer samples from control samples, achieving a sensitivity and specificity of 93.49 and 91.57%, respectively. In addition, we identified 3 of the 6 protein peaks at 2873, 6121 and 7778 m/z, which distinguished between stage I and stage II/III/IV gastric cancer. The model had an accuracy of 88.89% for the identification of stage I gastric cancer. In conclusion, the diagnostic model for the detection of serum proteins by SELDI-TOF-MS ProteinChip array technology correctly distinguishes gastric cancer from healthy samples, and has the ability to screen and distinguish between early gastric cancer from advanced gastric cancer.

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June 2012
Volume 5 Issue 6

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Li P, Zhang D and Guo C: Serum biomarker screening for the diagnosis of early gastric cancer using SELDI-TOF-MS. Mol Med Rep 5: 1531-1535, 2012.
APA
Li, P., Zhang, D., & Guo, C. (2012). Serum biomarker screening for the diagnosis of early gastric cancer using SELDI-TOF-MS. Molecular Medicine Reports, 5, 1531-1535. https://doi.org/10.3892/mmr.2012.834
MLA
Li, P., Zhang, D., Guo, C."Serum biomarker screening for the diagnosis of early gastric cancer using SELDI-TOF-MS". Molecular Medicine Reports 5.6 (2012): 1531-1535.
Chicago
Li, P., Zhang, D., Guo, C."Serum biomarker screening for the diagnosis of early gastric cancer using SELDI-TOF-MS". Molecular Medicine Reports 5, no. 6 (2012): 1531-1535. https://doi.org/10.3892/mmr.2012.834