No association between IRS‑1 promoter methylation and type 2 diabetes

Ma,Jiangbo; Cheng,Jia; Wang,Lingyan; Wang,Hongwei; Xu,Leiting; Liu,Panpan; Bu,Shizhong; Zhang,Lina; Le,Yanping; Ye,Meng; Wang,Qinwen; Shi,Yuping; Duan,Shiwei

doi:10.3892/mmr.2013.1569

No association between IRS‑1 promoter methylation and type 2 diabetes

Authors:
- Jiangbo Ma
- Jia Cheng
- Lingyan Wang
- Hongwei Wang
- Leiting Xu
- Panpan Liu
- Shizhong Bu
- Lina Zhang
- Yanping Le
- Meng Ye
- Qinwen Wang
- Yuping Shi
- Shiwei Duan
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Affiliations: Zhejiang Provincial People's Hospital, Hangzhou, Zhejiang 310014, P.R. China, The Affiliated Hospital, School of Medicine, Ningbo University, Ningbo, Zhejiang 315000, P.R. China, Bank of Blood Products, Ningbo No. 2 Hospital, Ningbo, Zhejiang 315010, P.R. China, Section of Endocrinology, The University of Chicago Pritzker School of Medicine, Chicago, IL 60637, USA, Zhejiang Provincial Key Laboratory of Pathophysiology, Ningbo University, Ningbo, Zhejiang 315211, P.R. China, Second Affiliated Hospital of Zhejiang University College of Medicine, Hangzhou, Zhejiang 310009, P.R. China
Published online on: July 3, 2013 https://doi.org/10.3892/mmr.2013.1569
Pages: 949-953

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Abstract

As a candidate gene for type 2 diabetes (T2D), insulin receptor substrate-1 (IRS‑1) gene variations were found to be associated with the risk of T2D. The aim of our study was to investigate the contribution of promoter DNA methylation of the IRS‑1 gene to the risk of T2D. Using bisulphite pyrosequencing technology, the DNA methylation levels of 3 CpG dinucleotides within the IRS‑1 gene promoter were measured in 48 T2D patients and 48 age‑ and gender‑matched healthy controls. Our results indicated that there was no significant association between the methylation of the IRS‑1 gene promoter and the risk of T2D (P>0.1). A breakdown analysis by gender revealed that IRS‑1 promoter methylation was not associated with an increased risk of T2D for either gender (P>0.1), although there were significantly lower methylation levels of CpG1 (P=0.002) and CpG2 (P=0.043) within the IRS‑1 gene promoter in males than in females.

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