Hwanggeumchal sorghum extract enhances BMP7 and GH signaling through the activation of Jak2/STAT5B 
in MC3T3‑E1 osteoblastic cells

Joung,Youn  Hee; Lim,Eun   Joung; Darvin,Pramod; Jang,Ju   Woong; Park,Kyung   Do; Lee,Hak  Kyo; Kim,Heui   Soo; Cho,Byung   Wook; Park,Taekyu; Chung,Sumi; Park,Jong   Hwan; Yang,Young  Mok

doi:10.3892/mmr.2013.1593

Hwanggeumchal sorghum extract enhances BMP7 and GH signaling through the activation of Jak2/STAT5B in MC3T3‑E1 osteoblastic cells

Authors:
- Youn Hee Joung
- Eun Joung Lim
- Pramod Darvin
- Ju Woong Jang
- Kyung Do Park
- Hak Kyo Lee
- Heui Soo Kim
- Byung Wook Cho
- Taekyu Park
- Sumi Chung
- Jong Hwan Park
- Young Mok Yang
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Affiliations: Department of Pathology, School of Medicine, Institute of Biomedical Science and Technology, Konkuk University, Seoul 143‑701, Republic of Korea, Korea Bone Bank Co, Ltd., Seoul 153‑782, Republic of Korea, Genomic Informatics Center, Hankyong National University, Anseong 456‑749, Republic of Korea, Department of Biological Sciences, College of Natural Sciences, Pusan National University, Busan 609‑735, Republic of Korea, Department of Animal Science, College of Life Science, Pusan National University, Busan 609‑735, Republic of Korea, Department of Biotechnology, College of Biomedical and Health Science, Konkuk University Glocal Campus, Chung‑Ju 380‑701, Republic of Korea, Department of Business, University of Texas at Austin, Austin, TX 78712, USA, College of Pharmacy, Dongguk University, Seoul 100-715, Republic of Korea
Published online on: July 18, 2013 https://doi.org/10.3892/mmr.2013.1593
Pages: 891-896

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Abstract

Sorghum is a principal cereal food in a number of parts of the world and is critical in folk medicine in Asia and Africa. However, its effects on bone are unknown. Growth hormone (GH) is a regulator of bone growth and bone metabolism. GH activates several signaling pathways, including the Janus kinase (Jak)/signal transducer and activator of transcription (STAT) pathways, thereby regulating expression of genes, including insulin‑like growth factor (IGF)‑1. Bone morphogenetic proteins (BMPs) induce the differentiation of cells of the osteoblastic lineage, increasing the pool of IGF‑1 target cells, the mature osteoblasts. In the present study, the effects of Hwanggeumchal sorghum extracts (HSE) on GH signaling via the Jak/STAT pathway in osteoblasts were investigated. HSE was not observed to be toxic to osteoblastic cells and increased the expression of BMP7 and GH‑related proteins, including STAT5B, p‑STAT5B, IGF‑1 receptor (IGF-1R), growth receptor hormone (GHR) and Jak2 in MC3T3‑E1 cells. In addition, HSE increased BMP7 and GHR mRNA expression in MC3T3‑E1 cells. The expression of HSE‑induced BMP7 and GHR was inhibited by AG490, a Jak2 kinase inhibitor. The observations indicate that HSE‑induced signaling is similar to GH signaling via the GHR‑Jak2 signaling axis. Using small interference RNA (siRNA) analysis, STAT5B was found to play an essential role in HSE‑induced BMP7 and GH signaling in MC3T3‑E1 cells. Results of the current study indicate that HSE promotes bone growth through activation of STAT5B.

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