Relevance of class 1 integrons and extended‑spectrum β‑lactamases in drug‑resistant Escherichia coli

Liu,Li‑Tao; Wan,Li‑Hong; Song,Xiao‑Hong; Xiong,Yao; Jin,Shao‑Ju; Zhou,Li‑Ming

doi:10.3892/mmr.2013.1626

Relevance of class 1 integrons and extended‑spectrum β‑lactamases in drug‑resistant Escherichia coli

Authors:
- Li‑Tao Liu
- Li‑Hong Wan
- Xiao‑Hong Song
- Yao Xiong
- Shao‑Ju Jin
- Li‑Ming Zhou
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Affiliations: Department of Pharmacology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu, Sichuan 610041, P.R. China, Department of Pharmacology, College of Pharmacy, Ningxia Medical University, Yinchuan 750004, P.R. China
Published online on: August 12, 2013 https://doi.org/10.3892/mmr.2013.1626
Pages: 1251-1255

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Abstract

Escherichia coli is a common cause of community‑ and hospital‑acquired urinary tract infections, and class 1 integrons are the prior elements of gene transference in the capture and distribution of gene cassettes among clinical gram-negative bacillus. In the present study, the resistance of Escherichia coli to antimicrobial agents was investigated. A total of 97 isolates were found to be susceptible to 16 antimicrobial agents and were detected in the production of extended β‑lactamases (ESBLs), distribution of CTX‑M‑type β‑lactamases, presence and characterization of class 1 integrons and a variable region of integron‑positive isolates. Escherichia coli isolates possessing CTX‑M (31; 32%) were detected in 19 isolates (61.5%). The presence of ESBLs was associated with resistance to penicillins, third-generation cephalosporins, ciprofloxacin, aminoglycosides and monocyclic β‑lactam antibiotics. Escherichia coli isolates (69; 71.1%) possessed class 1 integrons associated with resistance to ciprofloxacin and numerous third-generation cephalosporins, penicillins, tobramycin and trimethoprim‑sulfamethoxazole. The four gene cassette arrangements were as follows: dfrA17‑aadA5, aadA1, aacC4‑cmlA1 and dfr2d, and 8 carried two disparate class 1 integrons. Five isolates presented class 1 integrons containing no gene cassettes. The distribution of ESBLs and class 1 integrons in Escherichia coli were prevalent with drug resistance in Chengdu. In addition, the resistance range of Escherichia coli isolates that harboured ESBLs and carried class 1 integrons were similar. The current study demonstrated the presence of class 1 integrons and ESBLs, which jointly mediate the resistance of Escherichia coli isolates to a number of antibacterial agents.

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1	Sabharwal ER: Antibiotic susceptibility patterns of uropathogens in obstetric patients. N Am J Med Sci. 4:316–319. 2012. View Article : Google Scholar : PubMed/NCBI
2	Kang HY, Jeong YS, Oh JY, Tae SH, Choi CH, Moon DC, Lee WK, Lee YC, Seol SY, Cho DT and Lee JC: Characterization of antimicrobial resistance and class 1 integrons found in Escherichia coli isolates from humans and animals in Korea. J Antimicrob Chemother. 55:639–644. 2005. View Article : Google Scholar : PubMed/NCBI
3	Seputiené V, Povilonis J, Ruzauskas M, Pavilonis A and Suziedéliené E: Prevalence of trimethoprim resistance genes in Escherichia coli isolates of human and animal origin in Lithuania. J Med Microbiol. 59:315–322. 2010.
4	Xiao YH, Giske CG, Wei ZQ, Shen P, Heddini A and Li LJ: Epidemiology and characteristics of antimicrobial resistance in China. Drug Resist Updat. 14:236–250. 2011. View Article : Google Scholar : PubMed/NCBI
5	Jiang HX, Tang D, Liu YH, Zhang XH, Zeng ZL, Xu L and Hawkey PM: Prevalence and characteristics of β-lactamase and plasmid-mediated quinolone resistance genes in Escherichia coli isolated from farmed fish in China. J Antimicrob Chemother. 67:2350–2253. 2012.
6	Chen YH, Hsueh PR, Badal RE, Hawser SP, Hoban DJ, Bouchillon SK, Ni Y and Paterson DL: Antimicrobial susceptibility profiles of aerobic and facultative Gram-negative bacilli isolated from patients with intra-abdominal infections in the Asia-Pacific region according to currently established susceptibility interpretive criteria. J Infect. 62:280–291. 2011. View Article : Google Scholar
7	Wu H, Liu BG, Liu JH, Pan YS, Yuan L and Hu GZ: Phenotypic and molecular characterization of CTX-M-14 extended-spectrum β-lactamase and plasmid-mediated ACT-like AmpC β-lactamase produced by Klebsiella pneumoniae isolates from chickens in Henan Province, China. Genet Mol Res. 11:3357–3364. 2012.
8	Paterson DL and Bonomo RA: Extended-spectrum beta-lactamases: a clinical update. Clin Microbiol Rev. 18:657–686. 2005. View Article : Google Scholar : PubMed/NCBI
9	Han N, Sheng D and Xu H: Role of Escherichia coli strain subgroups, integrons, and integron-associated gene cassettes in dissemination of antimicrobial resistance in aquatic environments of Jinan, China. Water Sci Technol. 66:2385–2392. 2012.
10	Hall RM: Integrons and gene cassettes: hotspots of diversity in bacterial genomes. Ann NY Acad Sci. 1267:71–78. 2012. View Article : Google Scholar : PubMed/NCBI
11	Woodford N, Carattoli A, Karisik E, Underwood A, Ellington MJ and Livermore DM: Complete nucleotide sequences of plasmids pEK204, pEK499 and pEK516, encoding CTX-M enzymes in three major Escherichia coli lineages from the United Kingdom, all belonging to the international O25:H4-ST131 clone. Antimicrob Agents Chemother. 53:4472–4482. 2009. View Article : Google Scholar : PubMed/NCBI
12	Bennett PM: Plasmid encoded antibiotic resistance: acquisition and transfer of antibiotic resistance genes in bacteria. Br J Pharmacol. 153(Suppl 1): S347–S357. 2008. View Article : Google Scholar : PubMed/NCBI
13	Guérin E, Jové T, Tabesse A, Mazel D and Ploy MC: High-level gene cassette transcription prevents integrase expression in class 1 integrons. J Bacteriol. 193:5675–5682. 2011.PubMed/NCBI
14	van Essen-Zandbergen A, Smith H, Veldman K and Mevius D: Occurrence and characteristics of class 1, 2 and 3 integrons in Escherichia coli, Salmonella and Campylobacter spp. in the Netherlands. J Antimicrob Chemother. 59:746–750. 2007.PubMed/NCBI
15	Nardelli M, Scalzo PM, Ramírez MS, Quiroga MP, Cassini MH and Centrón D: Class 1 integrons in environments with different degrees of urbanization. PLoS One. 7:e392232012. View Article : Google Scholar : PubMed/NCBI
16	Clinical and Laboratory Standards Institute. Performance standards for antimicrobial susceptibility testing standards: nineteenth informational supplement M100-S19. 2009 Accessed January, 2013
17	Wu LT, Tsou MF, Wu HJ, Chen HE, Chuang YC and Yu WL: Survey of CTX-M-3 extended-spectrum beta-lactamase (ESBL) among cefotaxime-resistant Serratia marcescens at a medical center in middle Taiwan. Diagn Microbiol Infect Dis. 49:125–129. 2004. View Article : Google Scholar : PubMed/NCBI
18	Costa D, Poeta P, Sáenz Y, Coelho AC, Matos M, Vinué L, Rodrigues J and Torres C: Prevalence of antimicrobial resistance and resistance genes in faecal Escherichia coli isolates recovered from healthy pets. Vet Microbiol. 127:97–105. 2008. View Article : Google Scholar : PubMed/NCBI
19	Hsu SC, Chiu TH, Pang JC, Hsuan-Yuan CH, Chang GN and Tsen HY: Characterisation of antimicrobial resistance patterns and class 1 integrons among Escherichia coli and Salmonella enterica serovar Choleraesuis strains isolated from humans and swine in Taiwan. Int J Antimicrob Agents. 27:383–391. 2006. View Article : Google Scholar : PubMed/NCBI
20	Wu S, Dalsgaard A, Hammerum AM, Porsbo LJ and Jensen LB: Prevalence and characterization of plasmids carrying sulfonamide resistance genes among Escherichia coli from pigs, pig carcasses and human. Acta Vet Scand. 52:472010. View Article : Google Scholar : PubMed/NCBI
21	Lim KT, Yasin R, Yeo CC, Puthucheary S and Thong KL: Characterization of multidrug resistant ESBL-producing Escherichia coli isolates from hospitals in Malaysia. J Biomed Biotechnol. 2009:1656372009.PubMed/NCBI
22	Yang CM, Lin MF, Lin CH, Huang YT, Hsu CT and Liou ML: Characterization of antimicrobial resistance patterns and integrons in human fecal Escherichia coli in Taiwan. Jpn J Infect Dis. 62:177–181. 2009.PubMed/NCBI
23	Ozgumus OB, Celik-Sevim E, Alpay-Karaoglu S, Sandalli C and Sevim A: Molecular characterization of antibiotic resistant Escherichia coli strains isolated from tap and spring waters in a coastal region in Turkey. J Microbiol. 45:379–387. 2007.PubMed/NCBI
24	Birgy A, Cohen R, Levy C, Bidet P, Courroux C, Benani M, Thollot F and Bingen E: Community faecal carriage of extended-spectrum beta-lactamase-producing Enterobacteriaceae in french children. BMC Infect Dis. 12:3152012. View Article : Google Scholar : PubMed/NCBI
25	Ramirez MS, Parenteau TR, Centron D and Tolmasky ME: Functional characterization of Tn1331 gene cassettes. J Antimicrob Chemother. 62:669–673. 2008. View Article : Google Scholar : PubMed/NCBI
26	Bennett PM: Integrons and gene cassettes: a genetic construction kit for bacteria. J Antimicrob Chemother. 43:1–4. 1999. View Article : Google Scholar : PubMed/NCBI