Expression of neuronal protein Kidins220/ARMS in the spleen and peripheral blood of mice following airway allergen challenge

Li,Ning; Dong,Xu; Yang,Chang; Liu,Yuli; Ni,Xiuqin

doi:10.3892/mmr.2013.1727

Expression of neuronal protein Kidins220/ARMS in the spleen and peripheral blood of mice following airway allergen challenge

Authors:
- Ning Li
- Xu Dong
- Chang Yang
- Yuli Liu
- Xiuqin Ni
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Affiliations: Liaoning University of Tradional Chinese Medicine, Shenyang, Liaoning 116600, P.R. China, Department of Nephrology, Shenzhen Second People's Hospital, Shenzhen, Guangdong 518035, P.R. China, Department of Anatomy, Harbin Medical University at Daqing, Daqing, Heilongjiang 163319, P.R. China
Published online on: October 14, 2013 https://doi.org/10.3892/mmr.2013.1727
Pages: 1871-1875

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Abstract

Nerve growth factor (NGF), combined with the high‑affinity tyrosine kinase receptor A (TrkA), has been reported to be involved in the pathogenesis of asthma. Ankyrin‑rich membrane spanning/transmembrane substrate of protein kinase D (ARMS/Kidins220), a TrkA‑binding protein, modulates the NGF signaling pathway. The aim of the present study was to investigate the expression of Kidins220/ARMS and the effect NGF has on the protein in the spleen and peripheral blood, following airway allergen challenge in mice. BALB/c mice were sensitized and challenged with ovalbumin. The effects of NGF on Kidins220/ARMS in the spleen and peripheral blood of mice were assessed by administering anti-NGF antibody. Expression of ARMS, interleukin (IL)‑1β and IL‑4 in the spleen and peripheral blood was observed by reverse transcription‑polymerase chain reaction, western blot analysis and immunohistochemistry. Pathological changes in the bronchi and lung tissues were examined by hematoxylin and eosin staining. Results showed that Kidins220/ARMS, IL‑1β and IL‑4 were overexpressed in the spleen and peripheral blood following allergen challenge, compared with the control mice. Moreover, following treatment with anti-NGF, the levels of Kidins220/ARMS, IL‑1β and IL‑4 in the mice were downregulated. Therefore, the results of the present study showed that Kidins220/ARMS is expressed in the spleen and peripheral blood of normal BALB/c mice and may participate in the immuno‑inflammation of asthma through the NGF‑mediated signaling pathway.

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