MicroRNA-203 inhibits the proliferation and invasion of U251 glioblastoma cells by directly targeting PLD2 Retraction in /10.3892/mmr.2023.13154

Chen,Zigui; Li,Dazhi; Cheng,Quan; Ma,Zhiming; Jiang,Bing; Peng,Renjun; Chen,Rui; Cao,Yiqiang; Wan,Xin

doi:10.3892/mmr.2013.1814

MicroRNA-203 inhibits the proliferation and invasion of U251 glioblastoma cells by directly targeting PLD2

Retraction in: /10.3892/mmr.2023.13154

Authors:
- Zigui Chen
- Dazhi Li
- Quan Cheng
- Zhiming Ma
- Bing Jiang
- Renjun Peng
- Rui Chen
- Yiqiang Cao
- Xin Wan
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Affiliations: Department of Neurosurgery, The First Xiangya Hospital of Central South University, Changsha, Hunan 410008, P.R. China, Department of Neurosurgery, Traditional Chinese Medicine Hospital of Xinjiang Medical University, Urumchi, Xinjiang 830000, P.R. China
Published online on: November 20, 2013 https://doi.org/10.3892/mmr.2013.1814
Pages: 503-508

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Abstract

MicroRNAs (miRNAs) have been demonstrated to be important in the development and progression of various types of cancer. However, the exact roles of certain anti‑oncogenic miRNAs in human malignant gliomas remain to be elucidated. The present study aimed to reveal the expression of microRNA‑203 (miR-203) in normal brain tissues and gliomas, and to investigate the role of miR-203 in cell proliferation and migration in human glioblastoma U251 cells. Real-time reverse transcription polymerase chain reaction (RT-PCR) showed that the expression of miR-203 in high WHO grade glioma tissues was significantly decreased compared with low WHO grade glioma tissues and normal brain tissues, and its expression demonstrated a decreasing tendency with ascending WHO grades. The transfection of the miR-203 mimic into U251 cells markedly downregulated the expression of phospholipase D2 (PLD2), which was identified as a direct target of miR-203. Furthermore, miR-203 overexpression significantly suppressed the proliferation and invasion of U251 cells, while the overexpression of PLD2 abrogated these effects induced by the miR-203 mimic. In conclusion, the present study demonstrated the clinical significance of miR-203 in gliomas and suggested that miR-203 was able to inhibit the proliferation and invasion of glioma cells, partially at least via suppressing the protein expression of PLD2. Thus, miR-203 may be a novel candidate for the development of therapeutic strategies for gliomas.

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1	Surawicz TS, Davis F, Freels S, Laws ER Jr and Menck HR: Brain tumor survival: results from the National Cancer Data Base. J Neurooncol. 40:151–160. 1998. View Article : Google Scholar : PubMed/NCBI
2	Bondy ML, Scheurer ME, Malmer B, et al: Brain tumor epidemiology: consensus from the Brain Tumor Epidemiology Consortium. Cancer. 113:1953–1968. 2008. View Article : Google Scholar : PubMed/NCBI
3	Chargari C, Moncharmont C, Lévy A, et al: Cancer stem cells, cornerstone of radioresistance and perspectives for radiosensitization: glioblastoma as an example. Bull Cancer. 99:1153–1160. 2012.(In French).
4	Schepeler T: Emerging roles of microRNAs in the Wnt signaling network. Crit Rev Oncog. 18:357–371. 2013. View Article : Google Scholar : PubMed/NCBI
5	Yates LA, Norbury CJ and Gilbert RJ: The long and short of microRNA. Cell. 153:516–519. 2013. View Article : Google Scholar : PubMed/NCBI
6	Nikaki A, Piperi C and Papavassiliou AG: Role of microRNAs in gliomagenesis: targeting miRNAs in glioblastoma multiforme therapy. Expert Opin Investig Drugs. 21:1475–1488. 2012. View Article : Google Scholar : PubMed/NCBI
7	Profumo V and Gandellini P: MicroRNAs: cobblestones on the road to cancer metastasis. Crit Rev Oncog. 18:341–355. 2013. View Article : Google Scholar : PubMed/NCBI
8	Farazi TA, Hoell JI, Morozov P and Tuschl T: MicroRNAs in human cancer. Adv Exp Med Biol. 774:1–20. 2013. View Article : Google Scholar
9	Noguchi S, Mori T, Otsuka Y, et al: Anti-oncogenic microRNA-203 induces senescence by targeting E2F3 protein in human melanoma cells. J Biol Chem. 287:11769–11777. 2012. View Article : Google Scholar : PubMed/NCBI
10	Furuta M, Kozaki KI, Tanaka S, Arii S, Imoto I and Inazawa J: miR-124 and miR-203 are epigenetically silenced tumor-suppressive microRNAs in hepatocellular carcinoma. Carcinogenesis. 31:766–776. 2010. View Article : Google Scholar : PubMed/NCBI
11	Schetter AJ, Leung SY, Sohn JJ, et al: MicroRNA expression profiles associated with prognosis and therapeutic outcome in colon adenocarcinoma. JAMA. 299:425–436. 2008. View Article : Google Scholar : PubMed/NCBI
12	Boll K, Reiche K, Kasack K, et al: MiR-130a, miR-203 and miR-205 jointly repress key oncogenic pathways and are downregulated in prostate carcinoma. Oncogene. 32:277–285. 2013. View Article : Google Scholar : PubMed/NCBI
13	Yuan Y, Zeng ZY, Liu XH, et al: MicroRNA-203 inhibits cell proliferation by repressing DeltaNp63 expression in human esophageal squamous cell carcinoma. BMC Cancer. 11:572011. View Article : Google Scholar : PubMed/NCBI
14	He J, Deng Y, Yang G and Xie W: MicroRNA-203 down-regulation is associated with unfavorable prognosis in human glioma. J Surg Oncol. 108:121–125. 2013. View Article : Google Scholar : PubMed/NCBI
15	Bian K, Fan J, Zhang X, et al: MicroRNA-203 leads to G1 phase cell cycle arrest in laryngeal carcinoma cells by directly targeting survivin. FEBS Lett. 586:804–809. 2012. View Article : Google Scholar : PubMed/NCBI
16	Wang C, Zheng X, Shen C and Shi Y: MicroRNA-203 suppresses cell proliferation and migration by targeting BIRC5 and LASP1 in human triple-negative breast cancer cells. J Exp Clin Cancer Res. 31:582012. View Article : Google Scholar : PubMed/NCBI
17	Wei W, Wanjun L, Hui S, Dongyue C, Xinjun Y and Jisheng Z: miR-203 inhibits proliferation of HCC cells by targeting survivin. Cell Biochem Funct. 31:82–85. 2013. View Article : Google Scholar : PubMed/NCBI
18	Rani SB, Rathod SS, Karthik S, Kaur N, Muzumdar D and Shiras AS: MiR-145 functions as a tumor-suppressive RNA by targeting Sox9 and adducin 3 in human glioma cells. Neuro Oncol. 15:1302–1316. 2013. View Article : Google Scholar : PubMed/NCBI
19	He Z, Cen D, Luo X, et al: Downregulation of miR-383 promotes glioma cell invasion by targeting insulin-like growth factor 1 receptor. Med Oncol. 30:5572013. View Article : Google Scholar : PubMed/NCBI
20	Gao H, Zhao H and Xiang W: Expression level of human miR-34a correlates with glioma grade and prognosis. J Neurooncol. 113:221–228. 2013. View Article : Google Scholar : PubMed/NCBI
21	Wei T, Xu N, Meisgen F, Ståhle M, Sonkoly E and Pivarcsi A: Interleukin-8 is regulated by miR-203 at the posttranscriptional level in primary human keratinocytes. Eur J Dermatol. Apr 19–2013.(Epub ahead of print).
22	Abella V, Valladares M, Rodriguez T, et al: miR-203 regulates cell proliferation through its influence on Hakai expression. PLoS One. 7:e525682012. View Article : Google Scholar : PubMed/NCBI
23	Takeshita N, Mori M, Kano M, et al: miR-203 inhibits the migration and invasion of esophageal squamous cell carcinoma by regulating LASP1. Int J Oncol. 41:1653–1661. 2012.PubMed/NCBI
24	Szumiło M and Rahden-Staroń I: Phospholipase D in mammalian cells: structure, properties, physiological and pathological role. Postepy Hig Med Dosw (Online). 60:421–430. 2006.(In Polish).
25	Gomez-Cambronero J: Biochemical and cellular implications of a dual lipase-GEF function of phospholipase D2 (PLD2). J Leukoc Biol. 92:461–467. 2012. View Article : Google Scholar : PubMed/NCBI
26	Zhao Y, Ehara H, Akao Y, et al: Increased activity and intranuclear expression of phospholipase D2 in human renal cancer. Biochem Biophys Res Commun. 278:140–143. 2000. View Article : Google Scholar : PubMed/NCBI
27	Eisen SF and Brown HA: Selective estrogen receptor (ER) modulators differentially regulate phospholipase D catalytic activity in ER-negative breast cancer cells. Mol Pharmacol. 62:911–920. 2002. View Article : Google Scholar
28	Meacci E, Nuti F, Catarzi S, et al: Activation of phospholipase D by bradykinin and sphingosine 1-phosphate in A549 human lung adenocarcinoma cells via different GTP-binding proteins and protein kinase C delta signaling pathways. Biochemistry. 42:284–292. 2003. View Article : Google Scholar : PubMed/NCBI
29	Saito M, Iwadate M, Higashimoto M, Ono K, Takebayashi Y and Takenoshita S: Expression of phospholipase D2 in human colorectal carcinoma. Oncol Rep. 18:1329–1334. 2007.PubMed/NCBI
30	Snider AJ, Zhang Z, Xie Y and Meier KE: Epidermal growth factor increases lysophosphatidic acid production in human ovarian cancer cells: roles for phospholipase D2 and receptor transactivation. Am J Physiol Cell Physiol. 298:C163–C170. 2010. View Article : Google Scholar
31	O’Reilly MC, Scott SA, Brown KA, et al: Development of dual PLD1/2 and PLD2 selective inhibitors from a common 1,3,8-Triazaspiro[4.5]decane Core: discovery of Ml298 and Ml299 that decrease invasive migration in U87-MG glioblastoma cells. J Med Chem. 56:2695–2699. 2013.PubMed/NCBI
32	Choi HJ and Han JS: Overexpression of phospholipase D enhances Bcl-2 expression by activating STAT3 through independent activation of ERK and p38MAPK in HeLa cells. Biochim Biophys Acta. 1823:1082–1091. 2012. View Article : Google Scholar : PubMed/NCBI
33	Ahn BH, Park MH, Lee YH, Kwon TK and Min do S: Up-regulation of cyclooxygenase-2 by cobalt chloride-induced hypoxia is mediated by phospholipase D isozymes in human astroglioma cells. Biochim Biophys Acta. 1773:1721–1731. 2007. View Article : Google Scholar : PubMed/NCBI