Cytotoxicity study on SH‑SY5Y cells cultured at high glucose levels and treated with bupivacaine

Li,Yawen; Xu,Shiyuan; Zhang,Qingguo; Li,Le; Lai,Luying; Zheng,Ting; Su,Jiaoling; Yang,Naimei; Li,Yuantao

doi:10.3892/mmr.2013.1843

Cytotoxicity study on SH‑SY5Y cells cultured at high glucose levels and treated with bupivacaine

Authors:
- Yawen Li
- Shiyuan Xu
- Qingguo Zhang
- Le Li
- Luying Lai
- Ting Zheng
- Jiaoling Su
- Naimei Yang
- Yuantao Li
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Affiliations: Department of Anesthesia, Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong 510282, P.R. China, Department of Anesthesia, Shenzhen Maternal and Child Health Hospital, Southern Medical University, Shenzhen, Guangdong 510282, P.R. China
Published online on: December 6, 2013 https://doi.org/10.3892/mmr.2013.1843
Pages: 515-520

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Abstract

The aim of this study was to investigate susceptibility to the neurotoxicity of local anesthetic agents in a model of gestational diabetes mellitus (GDM). SH-SY5Y cells were cultured at different concentrations of glucose and subsequently treated with 1 mmol/l bupivacaine for 6 h. Reactive oxygen species (ROS) production and apoptosis were assessed using flow cytometry in each group of cells. The MTT method was utilized to detect cell survival, and western blot analysis was used to examine changes in 78 kDa glucose‑regulated protein (GRP78) levels in neuronal cells. In all groups, levels of ROS production, cell survival and GRP78 expression were significantly different (P<0.01) following the addition of various concentrations of glucose and bupivacaine, as well as for the interaction between different concentrations of the anesthetic agents, demonstrating a statistically significant difference. In conclusion, the susceptibility of SH-SY5Y cells to the neurotoxicity of local anesthetic agents was enhanced in a model of GDM.

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