miR-137 regulates the migration of human umbilical vein endothelial cells by targeting ephrin-type A receptor 7

Lu,Yucheng; Heng,Xueyuan; Yu,Jixu; Su,Quanping; Guan,Xianghong; You,Cuiping; Wang,Long; Che,Fengyuan

doi:10.3892/mmr.2014.2319

miR-137 regulates the migration of human umbilical vein endothelial cells by targeting ephrin-type A receptor 7

Authors:
- Yucheng Lu
- Xueyuan Heng
- Jixu Yu
- Quanping Su
- Xianghong Guan
- Cuiping You
- Long Wang
- Fengyuan Che
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Affiliations: Central Laboratory, Linyi People's Hospital, Shandong University, Linyi, Shandong 276000, P.R. China, Department of Neurosurgery, Linyi People's Hospital, Shandong University, Linyi, Shandong 276000, P.R. China, Department of Neurology, Linyi People's Hospital, Shandong University, Linyi, Shandong 276000, P.R. China
Published online on: June 13, 2014 https://doi.org/10.3892/mmr.2014.2319
Pages: 1475-1480

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Abstract

MicroRNAs (miRNAs) are short non-coding RNAs, which negatively regulate gene expression. Post‑transcriptional regulation by miRNAs is important for organism development. In addition, endothelial cells are key regulators of angiogenesis. By using the 3-(4,5-dimethylthiazol-2-yl)‑2,5‑diphenyltetrazolium bromide (MTT), migration and gelatin sponge-chorioallantoic membrane assays, it was demonstrated that when miR-137 was overexpressed, cell viability and migration decreased. In addition, it was observed that blocking endogenous miR-137 increased cell viability and migration. Bioinformatics analysis indicated that the 3'‑untranslated region (3'UTR) of the ephrin type-A receptor 7 (EPHA7) has a putative binding site for miR-137. miR-137 is able to directly bind to the EPHA7 3'UTR and negatively regulate the expression of EPHA7. miR-137 is also able to decrease the growth and migration of human umbilical vein endothelial cells (HUVECs). The identification of the function of miR-137 and its target gene EPHA7 in HUVECs may provide novel insights into the mechanisms of angiogenesis.

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