Photoprotective effect of arctiin against ultraviolet B-induced damage in HaCaT keratinocytes is mediated by microRNA expression changes

Cha,Hwa   Jun; Lee,Ghang  Tai; Lee,Kwang  Sik; Lee,Kun  Kook; Hong,Jin  Tae; Lee,Na  Kyeong; Kim,Soo-Yeon; Lee,Bo  Mi; An,In-Sook; Hahn,Hyung  Jin; Ahn,Kyu  Joong; Lee,Su-Jae; An,Sungkwan; Bae,Seunghee

doi:10.3892/mmr.2014.2326

Photoprotective effect of arctiin against ultraviolet B-induced damage in HaCaT keratinocytes is mediated by microRNA expression changes

Authors:
- Hwa Jun Cha
- Ghang Tai Lee
- Kwang Sik Lee
- Kun Kook Lee
- Jin Tae Hong
- Na Kyeong Lee
- Soo-Yeon Kim
- Bo Mi Lee
- In-Sook An
- Hyung Jin Hahn
- Kyu Joong Ahn
- Su-Jae Lee
- Sungkwan An
- Seunghee Bae
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Affiliations: Korea Institute for Skin and Clinical Sciences and Molecular-Targeted Drug Research Center, Konkuk University, Seoul 143-701, Republic of Korea, Songpa R&D Center, Coreana Cosmetics Co., Ltd., Cheonan, Chungcheongnam-do 330-833, Republic of Korea, College of Pharmacy and Medical Research Center, Chungbuk National University, Cheongju, Chungcheongbuk-do 361-763, Republic of Korea, Department of Dermatology, Konkuk University School of Medicine, Seoul 143-701, Republic of Korea, Department of Chemistry, Hanyang University, Seoul 133-791, Republic of Korea
Published online on: June 13, 2014 https://doi.org/10.3892/mmr.2014.2326
Pages: 1363-1370

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Abstract

Human keratinocytes are located in the outermost skin layer and thus particularly vulnerable to ultraviolet B (UVB) radiation exposure. Previous studies have focused on the cellular and molecular perspectives of UVB-induced keratinocyte damage. In the present study, it was demonstrated that pretreatment with the phytochemical arctiin, one of the lignin compounds, protects human HaCaT keratinocytes from UVB-mediated damage. Biochemical assays revealed that UVB-induced cytotoxicity and cell death were significantly reduced in arctiin-pretreated HaCaT cells. In addition, arctiin promoted the wound healing and DNA repair properties of keratinocytes. The photoprotective effects of arctiin were associated with changes in the expression levels of specific microRNAs (miRNAs) in HaCaT cells. A bioinformatics analysis demonstrated that the miRNAs were functionally involved in cancer, cell cycle, and Wnt and mitogen-activated protein kinase signaling pathways. In the present study, the results from the cellular and molecular assays demonstrated a novel role for arctiin in UVB protection in keratinocytes, which is mediated by miRNA responses and the suppression of UVB-induced cell death. Furthermore, arctiin is implicated as a potential chemopreventive agent through UVB protection of keratinocytes.

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