Evaluation of vascular grafts based on polyvinyl alcohol cryogels

Conconi,Maria  Teresa; Borgio,Luca; Di Liddo,Rosa; Sartore,Leonardo; Dalzoppo,Daniele; Amistà,Pietro; Lora,Silvano; Parnigotto,Pier  Paolo; Grandi,Claudio

doi:10.3892/mmr.2014.2348

Evaluation of vascular grafts based on polyvinyl alcohol cryogels

Authors:
- Maria Teresa Conconi
- Luca Borgio
- Rosa Di Liddo
- Leonardo Sartore
- Daniele Dalzoppo
- Pietro Amistà
- Silvano Lora
- Pier Paolo Parnigotto
- Claudio Grandi
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Affiliations: Department of Pharmaceutical and Pharmacological Sciences, University of Padua, Padua 35131, Italy, Department of Plastic and Reconstructive Surgery, University of Padua, Padua 35131, Italy, Department of Neuroradiology, Hospital of Rovigo, Rovigo 45100, Italy, Foundation for Biology and Regenerative Medicine, TES‑Tissue Engineering and Signaling (ONLUS), Padua 35131, Italy, Foundation for Biology and Regenerative Medicine, TES‑Tissue Engineering and Signaling (ONLUS), Padua 35131, Italy
Published online on: June 19, 2014 https://doi.org/10.3892/mmr.2014.2348
Pages: 1329-1334

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Abstract

The present study designed and developed blood vessel substitutes (BVSs) composed of polyvinyl alcohol (PVA) cryogels. The in vitro results demonstrated that the coating of the polymer with lyophilized decellularized vascular matrix (DVM) greatly enhanced the adhesion of human umbilical vein endothelial cells (HUVECs). However, when PVA̸DVM BVSs were implanted into the abdominal aorta of Sprague‑Dawley rats, DVM was identified as a highly thrombogenic surface resulting in the mortality of all animals 3‑4 days after surgery. By contrast, all rats implanted with PVA survived and were sacrificed after 12 months. The luminal surface of the explanted grafts was completely covered by endothelial cells and the inner diameter was similar to that of the original vessel. In conclusion, the present study indicated that PVA may be considered as a promising biomaterial for the fabrication of artificial vessels.

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