Open Access

Tumor necrosis factor-α induces interleukin-34 expression through nuclear factor‑κB activation in MC3T3-E1 osteoblastic cells

  • Authors:
    • Yaqiong Yu
    • Di Yang
    • Lihong Qiu
    • Hirohiko Okamura
    • Jiajie Guo
    • Tatsuji Haneji
  • View Affiliations

  • Published online on: June 25, 2014     https://doi.org/10.3892/mmr.2014.2353
  • Pages: 1371-1376
  • Copyright: © Yu et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].

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Abstract

Osteoblasts produce various types of cytokines under pathological conditions and control osteoclast differentiation. Tumor necrosis factor-α (TNF-α) has been demonstrated to exert complex effects in osteoblasts under local inflammatory conditions, including in periodontal and periapical diseases. Interleukin-34 (IL-34) has been recently identified as a novel regulatory factor for the differentiation and function of osteoclasts. The present study provides the first evidence, to the best of our knowledge, that the expression of IL-34 is induced by TNF-α through nuclear factor-κB (NF-κB) activation in MC3T3-E1 osteoblastic cells. TNF-α induced IL-34 expression in a dose- and time-dependent manner. Immunocytochemistry with an NF-κB antibody demonstrated that NF-κB was mainly localized in the cytoplasm of the untreated MC3T3-E1 cells. Rapid translocation of NF-κB from the cytoplasm to the nucleus was observed in the cells treated with TNF-α for 15 min. Translocation and transcriptional activity of NF-κB were also determined by western blotting and a luciferase reporter assay, respectively. Pretreatment with 100 µM CAPE, an inhibitor of NF-κB, significantly inhibited TNF-α-induced IL-34 expression. These results indicate that TNF-α induces IL-34 expression via NF-κB in osteoblasts.
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September-2014
Volume 10 Issue 3

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Yu Y, Yang D, Qiu L, Okamura H, Guo J and Haneji T: Tumor necrosis factor-α induces interleukin-34 expression through nuclear factor‑κB activation in MC3T3-E1 osteoblastic cells. Mol Med Rep 10: 1371-1376, 2014
APA
Yu, Y., Yang, D., Qiu, L., Okamura, H., Guo, J., & Haneji, T. (2014). Tumor necrosis factor-α induces interleukin-34 expression through nuclear factor‑κB activation in MC3T3-E1 osteoblastic cells. Molecular Medicine Reports, 10, 1371-1376. https://doi.org/10.3892/mmr.2014.2353
MLA
Yu, Y., Yang, D., Qiu, L., Okamura, H., Guo, J., Haneji, T."Tumor necrosis factor-α induces interleukin-34 expression through nuclear factor‑κB activation in MC3T3-E1 osteoblastic cells". Molecular Medicine Reports 10.3 (2014): 1371-1376.
Chicago
Yu, Y., Yang, D., Qiu, L., Okamura, H., Guo, J., Haneji, T."Tumor necrosis factor-α induces interleukin-34 expression through nuclear factor‑κB activation in MC3T3-E1 osteoblastic cells". Molecular Medicine Reports 10, no. 3 (2014): 1371-1376. https://doi.org/10.3892/mmr.2014.2353