Bushen Huoxue decoction improves cognitive decline in rats with cerebral hypoperfusion

Ye,Shuifen; Gu,Yong; Xu,Yihui; Fan,Wen; Wang,Xiaoting; Chen,Shuting; Cai,Siyi; Lv,Shirong; Tong,Yao; Cai,Jing

doi:10.3892/mmr.2014.2355

Bushen Huoxue decoction improves cognitive decline in rats with cerebral hypoperfusion

Authors:
- Shuifen Ye
- Yong Gu
- Yihui Xu
- Wen Fan
- Xiaoting Wang
- Shuting Chen
- Siyi Cai
- Shirong Lv
- Yao Tong
- Jing Cai
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Affiliations: Academy of Integrative Medicine Fujian, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350122, P.R. China, School of Chinese Medicine, The University of Hong Kong, Hong Kong SAR, P.R. China, College of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350122, P.R. China
Published online on: June 26, 2014 https://doi.org/10.3892/mmr.2014.2355
Pages: 1635-1641

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Abstract

Cerebral hypoperfusion is a common feature of vascular dementia and has recently been recognized to contribute to the progression of cognitive decline. The present study aimed to investigate the effects of Bushen Huoxue decoction (BHD), a two‑herb Chinese Medicine, on cognitive impairment in a rat model of cerebral hypoperfusion induced by permanent occlusion of bilateral common carotid arteries (2VO). The results demonstrated that BHD significantly attenuated learning and spatial memory deficits in the Morris water maze test in a dose‑dependent manner. Transmission electron microscopy observation revealed that the reduction of synapse density in hippocampal CA1 and cortex parietal isolated from rats with 2VO was partially restored by BHD treatment. In addition, the expression levels of a number of antioxidants, including superoxide dismutase, catalase (CAT), glutathine and glutathione peroxidase‑1 (GPx‑1) increased, whereas malondialdehyde decreased in the hippocampi of rats with 2VO following BHD treatment. Polymerase chain reaction and western blot analysis further confirmed that the GPx‑1 and CAT expression increased in the BHD treatment group. In conclusion the results suggested that BHD has therapeutic potential to treat vascular dementia, which may be associated with synapse density and anti‑oxidant activities in the hippocampus.

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