MicroRNA-21, induced by high glucose, modulates macrophage apoptosis via programmed cell death 4

  • Authors:
    • Yuan‑Yuan Shang
    • Ning‑Ning Fang
    • Feng Wang
    • Hui Wang
    • Zhi‑Hao Wang
    • Meng‑Xiong Tang
    • Jie Peng
    • Yun Zhang
    • Wei Zhang
    • Ming Zhong
  • View Affiliations

  • Published online on: February 27, 2015     https://doi.org/10.3892/mmr.2015.3398
  • Pages: 463-469
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Abstract

MicroRNA‑21 (miR‑21) has been found to promote cell proliferation and survival. It has also been shown to exhibit an increased expression in a number of forms of cardiovascular disease. However, the mechanisms underlying the involvement of miR‑21 in atherosclerosis remain to be elucidated. In the present study, it was demonstrated that miR‑21 was upregulated in a time‑dependent manner in response to high‑concentration glucose stimulation in Raw 264.7 macrophages. High concentrations of glucose induce macrophage apoptosis. miR‑21‑inhibited macrophages treated with a normal concentration of glucose exhibited increased levels of cell apoptosis and augmented levels of activated caspase‑3, while cells treated with an miR‑21 inhibitor and a high concentration of glucose, revealed significantly increased levels of apoptosis. In addition, inhibition of miR‑21 increased mRNA and protein levels of programmed cell death 4 (PDCD4), which, by contrast, were reduced in miR‑21‑inhibited cells that had been treated with a high concentration of glucose. In conclusion, miR‑21 is sensitive to high‑concentration glucose treatment in macrophages, and appears to have a protective effect in macrophage apoptosis induced by high concentrations of glucose via PDCD4.
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July-2015
Volume 12 Issue 1

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Shang YY, Fang NN, Wang F, Wang H, Wang ZH, Tang MX, Peng J, Zhang Y, Zhang W, Zhong M, Zhong M, et al: MicroRNA-21, induced by high glucose, modulates macrophage apoptosis via programmed cell death 4. Mol Med Rep 12: 463-469, 2015
APA
Shang, Y., Fang, N., Wang, F., Wang, H., Wang, Z., Tang, M. ... Zhong, M. (2015). MicroRNA-21, induced by high glucose, modulates macrophage apoptosis via programmed cell death 4. Molecular Medicine Reports, 12, 463-469. https://doi.org/10.3892/mmr.2015.3398
MLA
Shang, Y., Fang, N., Wang, F., Wang, H., Wang, Z., Tang, M., Peng, J., Zhang, Y., Zhang, W., Zhong, M."MicroRNA-21, induced by high glucose, modulates macrophage apoptosis via programmed cell death 4". Molecular Medicine Reports 12.1 (2015): 463-469.
Chicago
Shang, Y., Fang, N., Wang, F., Wang, H., Wang, Z., Tang, M., Peng, J., Zhang, Y., Zhang, W., Zhong, M."MicroRNA-21, induced by high glucose, modulates macrophage apoptosis via programmed cell death 4". Molecular Medicine Reports 12, no. 1 (2015): 463-469. https://doi.org/10.3892/mmr.2015.3398