Effect of β-anhydroicaritin on the expression levels of tumor necrosis factor-α and matrix metalloproteinase-3 in periodontal tissue of diabetic rats

Wu,Yingtao; Wang,Wanchun; Liu,Lian

doi:10.3892/mmr.2015.3591

Effect of β-anhydroicaritin on the expression levels of tumor necrosis factor-α and matrix metalloproteinase-3 in periodontal tissue of diabetic rats

Authors:
- Yingtao Wu
- Wanchun Wang
- Lian Liu
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Affiliations: Department of Periodontology and Oral Mucosa Diseases, Qingdao Stomatological Hospital, Qingdao, Shandong 266001, P.R. China, Department of Acupuncture and Moxibustion, Qingdao Hiser Medical Group, Qingdao, Shandong 266001, P.R. China
Published online on: April 2, 2015 https://doi.org/10.3892/mmr.2015.3591
Pages: 1829-1837
Copyright: © Wu et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].

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Abstract

The present study aimed to investigate the effect of β-anhydroicaritin on the expression levels of tumor necrosis factor (TNF)-α and matrix metalloproteinase (MMP)-3, and the pathological changes in the periodontal tissue of diabetic rats. Male Wistar rats (n=40; three months old) were randomly divided into four groups: Normal control group, diabetes group, diabetes + β‑anhydroicaritin group and diabetes + urate group, (n=10 in each group). Following an overnight fast, diabetes was induced by intraperitoneal injection of streptozocin. The rats were maintained for 12 weeks and the blood sugar, urine sugar and body weight were assessed in week 12. Histological changes of the periodontal tissues were observed by hematoxylin and eosin staining, and the expression levels of TNF‑α and MMP‑3 were observed by immunohistochemistry. Following 12 weeks, the TNF‑α grey value in the diabetes group was significantly lower compared with that in the control group (P<0.05), while no significant difference was observed between TNF‑α levels in the diabetes + β‑anhydroicaritin group, diabetes + urate group and the control group (P>0.05). However, TNF‑α levels in the diabetes + β‑anhdroicaritin group and diabetes + urate group were significantly higher compared with those in the diabetes group (P<0.05), and those in the diabetes + β‑anhydroicaritin group were lower compared with those in the diabetes + urate group (P<0.05). The MMP‑3 grey value in the diabetes group was significantly lower compared with that in the control group (P<0.05), while no significant difference was observed between MMP‑3 levels in the diabetes + β‑anhydroicaritin group, diabetes + urate group and the control group (P>0.05). However, MMP‑3 levels the diabetes + β‑anhydroicaritin group and diabetes + urate group were significantly higher compared with those in the diabetes group (P<0.05), and those in the diabetes + β‑anhydroicaritin group were lower compared with those in the diabetes + urate group (P<0.01). β‑anhydroicaritin normalized the expression levels of TNF‑α and MMP‑3 in the periodontal tissue of diabetic rats and led to the recovery of the changes in the morphological structure of the periodontal tissue.

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1	Xu JL, Meng HX, Li Z, Li FY and Zhang L: Effect of periodontal therapy on metabolic control and serum biochemical markers in subjects with type 2 diabetes and chronic periodontitis. Beijing Da Xue Xue Bao. 45:27–32. 2013.In Chinese. PubMed/NCBI
2	Schara R, Skaleric E, Seme K and Skaleric U: Prevalence of periodontal pathogens and metabolic control of type 1 diabetes patients. J Int Acad Periodontol. 15:29–34. 2013.PubMed/NCBI
3	Bascones-Martínez A, Arias-Herrera S, Criado-Cámara E, Bascones-Ilundáin J and Bascones-Ilundáin C: Periodontal disease and diabetes. Adv Exp Med Biol. 771:76–87. 2012.
4	Li H, Li W, Ding Y, et al: Affect of protein tyrosine phosphatase non-receptor type 2 and nuclear factor-kappaB on periodontal destruction with diabetes. Hua Xi Kou Qiang Yi Xue Za Zhi. 30:598–602. 2012.In Chinese.
5	Al-Khabbaz AK and Al-Shammari KF: Diabetes mellitus and periodontal health: dentists’ knowledge. Med Princ Pract. 20:538–544. 2011. View Article : Google Scholar
6	Chen L, Luo G, Xuan D, et al: Effects of non-surgical periodontal treatment on clinical response, serum inflammatory parameters and metabolic control in patients with type 2 diabetes: a randomized study. J Periodontol. 83:435–443. 2012. View Article : Google Scholar
7	Silva JA, Ferrucci DL, Peroni LA, et al: Sequential IL-23 and IL-17 and increased Mmp8 and Mmp14 expression characterize the progression of an experimental model of periodontal disease in type 1 diabetes. J Cell Physiol. 227:2441–2450. 2012. View Article : Google Scholar
8	Cai L, Wang J, Li Y, Sun X, Wang L, Zhou Z and Kang YJ: Inhibition of superoxide generation and associated nitrosative damage is involved in metallothionein prevention of diabetic cardiomyopathy. Diabetes. 54:1829–1837. 2005. View Article : Google Scholar : PubMed/NCBI
9	Lee WH, Gounarides JS, Roos ES and Wolin MS: Influence of peroxynitrite on energy metabolism and cardiac function in a rat ischemia-reperfusion model. Am J Physiol Heart Circ Physiol. 285:H1385–H1395. 2003. View Article : Google Scholar : PubMed/NCBI
10	Nassar H, Kantarci A and van Dyke TE: Diabetic periodontitis: A model for activated innate immunity and impaired resolution of inflammation. Periodontol 2000. 43:233–244. 2007. View Article : Google Scholar : PubMed/NCBI
11	Mahadev K, Wu X, Zilbering A, Zhu L, Lawrence JT and Goldstein BJ: Hydrogen peroxide generated during cellular insulin stimulation is integral to activation of the distal insulin signaling cascade in 3T3-L1 adipocytes. J Biol Chem. 276:48662–48669. 2001. View Article : Google Scholar : PubMed/NCBI
12	Soares AF, Carvalho RA, Veiga FJ, et al: Restoration of direct pathway glycogen synthesis flux in the STZ-diabetes rat model by insulin administration. Am J Physiol Endocrinol Metab. 303:E875–E885. 2012. View Article : Google Scholar : PubMed/NCBI
13	Pacios S, Andriankaja O, Kang J, et al: Bacterial infection increases periodontal bone loss in diabetic rats through enhanced apoptosis. Am J Pathol. 183:1928–1935. 2013. View Article : Google Scholar : PubMed/NCBI
14	Xiong X, Elkind-Hirsch KE, Xie Y, et al: Periodontal disease as a potential risk factor for the development of diabetes in women with a prior history of gestational diabetes mellitus. J Public Health Dent. 73:41–49. 2013. View Article : Google Scholar
15	Almeida Abdo J, Cirano FR, Casati MZ, et al: Influence of dyslipidemia and diabetes mellitus on chronic periodontal disease. J Periodontol. 84:1401–1408. 2013. View Article : Google Scholar
16	Sgolastra F, Severino M, Pietropaoli D, Gatto R and Monaco A: Effectiveness of periodontal treatment to improve metabolic control in patients with chronic periodontitis and type 2 diabetes: a meta-analysis of randomized clinical trials. J Periodontol. 84:958–973. 2013. View Article : Google Scholar
17	Daniel R, Gokulanathan S, Shanmugasundaram N, Lakshmigandhan M and Kavin T: Diabetes and periodontal disease. J Pharm Bioallied Sci. 4(Suppl 2): S280–S282. 2012. View Article : Google Scholar : PubMed/NCBI
18	Monea A, Mezei T and Monea M: The influence of diabetes mellitus on periodontal tissues: a histological study. Rom J Morphol Embryol. 53:491–495. 2012.PubMed/NCBI
19	Al-Khabbaz AK, Al-Shammari KF, Hasan A and Abdul-Rasoul M: Periodontal health of children with type 1 diabetes mellitus in Kuwait: a case-control study. Med Princ Pract. 22:144–149. 2013. View Article : Google Scholar
20	Oates TW: Gingival blood glucose may screen for type 2 diabetes in patients with periodontal disease. J Evid Based Dent Pract. 12:154–155. 2012. View Article : Google Scholar : PubMed/NCBI
21	Obradović R, Kesić L, Mihailović D, Jovanović G, Antić S and Brkić Z: Low-level lasers as an adjunct in periodontal therapy in patients with diabetes mellitus. Diabetes Technol Ther. 14:799–803. 2012. View Article : Google Scholar
22	Boissonneault G; CHAC: Diabetes: exploring a periodontal connection. JAAPA. 25:18–20. 2012.PubMed/NCBI
23	Haseeb M, Khawaja KI, Ataullah K, Munir MB and Fatima A: Periodontal disease in type 2 diabetes mellitus. J Coll Physicians Surg Pak. 22:514–518. 2012.PubMed/NCBI
24	Demmer RT, Holtfreter B, Desvarieux M, et al: The influence of type 1 and type 2 diabetes on periodontal disease progression: prospective results from the study of health in Pomerania (SHIP). Diabetes Care. 35:2036–2042. 2012. View Article : Google Scholar : PubMed/NCBI
25	Bastos AS, Graves DT, Loureiro AP, et al: Lipid peroxidation is associated with the severity of periodontal disease and local inflammatory markers in patients with type 2 diabetes. J Clin Endocrinol Metab. 97:E1353–E1362. 2012. View Article : Google Scholar : PubMed/NCBI
26	Chang PC, Chien LY, Yeo JF, et al: Progression of periodontal destruction and the roles of advanced glycation end products in experimental diabetes. J Periodontol. 84:379–388. 2013. View Article : Google Scholar
27	Yoon AJ, Cheng B, Philipone E, Turner R and Lamster IB: Inflammatory biomarkers in saliva: assessing the strength of association of diabetes mellitus and periodontal status with the oral inflammatory burden. J Clin Periodontol. 39:434–440. 2012. View Article : Google Scholar : PubMed/NCBI
28	Rajhans NS, Kohad RM, Chaudhari VG and Mhaske NH: A clinical study of the relationship between diabetes mellitus and periodontal disease. J Indian Soc Periodontol. 15:388–392. 2011. View Article : Google Scholar
29	Sjödin B, Edblad E, Sondell K and Dahlén G: Minor manifestations of periodontal diseases in young adults with type 1 diabetes mellitus. Periodontal and microbiological findings. Acta Odontol Scand. 70:589–596. 2012. View Article : Google Scholar : PubMed/NCBI
30	Silva JA, Lopes Ferrucci D, Peroni LA, et al: Periodontal disease-associated compensatory expression of osteoprotegerin is lost in type 1 diabetes mellitus and correlates with alveolar bone destruction by regulating osteoclastogenesis. Cells Tissues Organs. 196:137–150. 2012. View Article : Google Scholar : PubMed/NCBI
31	Franek E, Januszkiewicz-Caulier J, Blach A, et al: Intima-media thickness and other markers of atherosclerosis in patients with type 2 diabetes and periodontal disease. Kardiol Pol. 70:7–13. 2012.PubMed/NCBI
32	Botero JE, Yepes FL, Roldán N, et al: Tooth and periodontal clinical attachment loss are associated with hyperglycemia in patients with diabetes. J Periodontol. 83:1245–1250. 2012. View Article : Google Scholar : PubMed/NCBI
33	Obradović R, Kesić L, Mihailović D, et al: A histological evaluation of a low-level laser therapy as an adjunct to periodontal therapy in patients with diabetes mellitus. Lasers Med Sci. 28:19–24. 2013. View Article : Google Scholar
34	Field CA, Gidley MD, Preshaw PM and Jakubovics N: Investigation and quantification of key periodontal pathogens in patients with type 2 diabetes. J Periodontal Res. 47:470–478. 2012. View Article : Google Scholar : PubMed/NCBI
35	Nassar PO, Walker CS, Salvador CS, Felipetti FA, Orrico SR and Nassar CA: Lipid profile of people with diabetes mellitus type 2 and periodontal disease. Diabetes Res Clin Pract. 96:35–39. 2012. View Article : Google Scholar
36	Lakschevitz F, Aboodi G, Tenenbaum H and Glogauer M: Diabetes and periodontal diseases: interplay and links. Curr Diabetes Rev. 7:433–439. 2011. View Article : Google Scholar : PubMed/NCBI