Diterpenoid B derived from Plectranthus excisus inhibits the melanoma cell cycle in the B16 melanoma cell line

Liu,Ya‑Nan; Gu,Jun‑Lian; Ma,Meng‑Shi; Guo,Hua; Liu,Long; Guo,Li‑Rong; Wang,Yue; Li,Yang

doi:10.3892/mmr.2015.3986

Diterpenoid B derived from Plectranthus excisus inhibits the melanoma cell cycle in the B16 melanoma cell line

Authors:
- Ya‑Nan Liu
- Jun‑Lian Gu
- Meng‑Shi Ma
- Hua Guo
- Long Liu
- Li‑Rong Guo
- Yue Wang
- Yang Li
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Affiliations: Department of Pathophysiology, Key Laboratory of Pathobiology, Ministry of Education, College of Basic Medical Sciences, Jilin University, Changchun, Jilin 130021, P.R. China, Department of Cardiovascular Medicine, China‑Japan Union Hospital, Jilin University, Changchun, Jilin 130033, P.R. China
Published online on: June 23, 2015 https://doi.org/10.3892/mmr.2015.3986
Pages: 4578-4583

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Abstract

Plectranthus excisus is widely distributed throughout northeast China. Its active ingredient, diterpenoids, exhibits significant antitumor effects. The present study examined the antitumor effects of diterpenoid B (DB), derived from Plectranthus excisus, and demonstrated that DB inhibited the proliferation of tumor cells by inhibiting the cell cycle. Reverse transcription‑quantitative polymerase chain reaction and western blot analysis were used to determine mRNA and protein expression levels, respectively. The results revealed that exposure to DB increased the expression levels of the transformation associated, protein 53, and cyclin‑dependent kinase inhibitor 1A, and decreased the expression of cyclin‑dependent kinase 2. The results of the present study demonstrated that DB can inhibit cell cycle progression and, therefore, offers potential as a beneficial antitumor drug.

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