NR2F2 regulates bone marrow-derived mesenchymal stem cell-promoted proliferation of Reh cells

  • Authors:
    • Ni Zhu
    • Huafang Wang
    • Jieping Wei
    • Binsheng Wang
    • Wei Shan
    • Xiaoyu Lai
    • Yanmin Zhao
    • Jian Yu
    • He Huang
  • View Affiliations

  • Published online on: June 10, 2016     https://doi.org/10.3892/mmr.2016.5389
  • Pages: 1351-1356
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Abstract

Bone marrow-derived mesenchymal stem cells (BM-MSCs) are pivotal components of the leukemic microenvironment. BM-MSCs have been previously reported to promote the proliferation of leukemic cells. To further understand the molecular mechanisms of BM-MSC-induced proliferation of leukemic cells, the present study co-cultured acute lymphoblastic leukemia (ALL) Reh cells with BM-MSCs. The current study used methods including shRNA, flow cytometry, MTT, reverse transcription-quantitative polymerase chain reaction, ELISA and western blotting. The data of the present study demonstrated that BM‑MSCs promote the proliferation of Reh cells and the NR2F2 mRNA and protein levels were elevated in BM‑MSCs following co‑culture. Additionally, it was demonstrated that shRNA knockdown of NR2F2 inhibited BM‑MSC‑induced proliferation of Reh cells. Furthermore, following downregulation of NR2F2, vascular endothelial growth factor A (VEGFA) secretion by BM‑MSCs was reduced. The present study demonstrated that NR2F2 mediates BM‑MSC‑induced proliferation of Reh cells, partially via regulation of VEGFA. Disrupting microenvironmental support by targeting NR2F2 may be a potential therapeutic strategy for ALL.
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August-2016
Volume 14 Issue 2

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Zhu N, Wang H, Wei J, Wang B, Shan W, Lai X, Zhao Y, Yu J and Huang H: NR2F2 regulates bone marrow-derived mesenchymal stem cell-promoted proliferation of Reh cells. Mol Med Rep 14: 1351-1356, 2016.
APA
Zhu, N., Wang, H., Wei, J., Wang, B., Shan, W., Lai, X. ... Huang, H. (2016). NR2F2 regulates bone marrow-derived mesenchymal stem cell-promoted proliferation of Reh cells. Molecular Medicine Reports, 14, 1351-1356. https://doi.org/10.3892/mmr.2016.5389
MLA
Zhu, N., Wang, H., Wei, J., Wang, B., Shan, W., Lai, X., Zhao, Y., Yu, J., Huang, H."NR2F2 regulates bone marrow-derived mesenchymal stem cell-promoted proliferation of Reh cells". Molecular Medicine Reports 14.2 (2016): 1351-1356.
Chicago
Zhu, N., Wang, H., Wei, J., Wang, B., Shan, W., Lai, X., Zhao, Y., Yu, J., Huang, H."NR2F2 regulates bone marrow-derived mesenchymal stem cell-promoted proliferation of Reh cells". Molecular Medicine Reports 14, no. 2 (2016): 1351-1356. https://doi.org/10.3892/mmr.2016.5389