Mechanism of uncoupling protein 2‑mediated myocardial injury in hypothermic preserved rat hearts

  • Authors:
    • Gai‑Ge Chen
    • Jin‑Bin Yan
    • Xu‑Ming Wang
    • Ming‑Zhi Zheng
    • Jian‑Ping Jiang
    • Xin‑Mei Zhou
    • Bin Cai
    • Yue‑Liang Shen
  • View Affiliations

  • Published online on: June 23, 2016     https://doi.org/10.3892/mmr.2016.5436
  • Pages: 1857-1864
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Abstract

In the present study, the alterations in uncoupling protein 2 (UCP2) expression following hypothermic preservation in rat hearts were investigated. Isolated rat hearts were preserved in Celsior solution for 3‑12 h followed by 60 min of reperfusion. The cardiac function was evaluated using the Langendorff perfusion system. UCP2 and silent mating type information regulation 2 homolog 1 (SIRT1) proteins were detected by western blot analysis. The ATP production and mitochondrial reactive oxygen species (ROS) levels were assessed. Subsequent to preservation in ice‑cold Celsior solution for 3‑12 h, the UCP2 protein expression in rat hearts was observed to increase in a time‑dependent manner. The UCP2 inhibitor genipin inhibited the hypothermic preservation‑induced cardiac dysfunction, prevented a decline in ATP production induced by 9 h of preservation, however had no effect on the hypothermic preservation‑induced increase in mitochondrial ROS levels. Compared with the control group, the SIRT1 protein expression in rat hearts reduced following hypothermic preservation. Compared with the 9‑h preservation group, Celsior solution supplemented with the SIRT1 activator resveratrol (20 or 40 µmol/l) inhibited UCP2 protein overexpression, prevented the decline in ATP production and resulted in an improvement cardiac function. The SIRT1 inhibitor EX‑527 abolished the resveratrol‑induced inhibition of UCP2 overexpression and cardiac protection in the hypothermic preserved rat heart. These observations suggest that downregulation of UCP2 expression in the hypothermic preserved rat heart in part initiated the protective mechanism via the SIRT1 pathway.
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August-2016
Volume 14 Issue 2

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Chen GG, Yan JB, Wang XM, Zheng MZ, Jiang JP, Zhou XM, Cai B and Shen YL: Mechanism of uncoupling protein 2‑mediated myocardial injury in hypothermic preserved rat hearts. Mol Med Rep 14: 1857-1864, 2016.
APA
Chen, G., Yan, J., Wang, X., Zheng, M., Jiang, J., Zhou, X. ... Shen, Y. (2016). Mechanism of uncoupling protein 2‑mediated myocardial injury in hypothermic preserved rat hearts. Molecular Medicine Reports, 14, 1857-1864. https://doi.org/10.3892/mmr.2016.5436
MLA
Chen, G., Yan, J., Wang, X., Zheng, M., Jiang, J., Zhou, X., Cai, B., Shen, Y."Mechanism of uncoupling protein 2‑mediated myocardial injury in hypothermic preserved rat hearts". Molecular Medicine Reports 14.2 (2016): 1857-1864.
Chicago
Chen, G., Yan, J., Wang, X., Zheng, M., Jiang, J., Zhou, X., Cai, B., Shen, Y."Mechanism of uncoupling protein 2‑mediated myocardial injury in hypothermic preserved rat hearts". Molecular Medicine Reports 14, no. 2 (2016): 1857-1864. https://doi.org/10.3892/mmr.2016.5436