Tristetraprolin regulates the decay of the hypoxia-induced vascular endothelial growth factor mRNA in ARPE-19 cells

  • Authors:
    • Jinhyun Ryu
    • Hyemin Seong
    • Nal Ae Yoon
    • Seong Wook Seo
    • Jeong Woo Park
    • Sang Soo Kang
    • Jong Moon Park
    • Yong Seop Han
  • View Affiliations

  • Published online on: October 26, 2016     https://doi.org/10.3892/mmr.2016.5890
  • Pages: 5395-5400
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Abstract

The aim of the present study was to investigate the effects of tristetraprolin (TTP) on the vascular endothelial growth factor (VEGF) mRNA and protein expression levels in retinal pigment epithelial cells under hypoxic conditions, and to consider the possibility of using TTP as a novel treatment tool for neovascular age‑related macular degeneration (AMD). Overexpression of TTP reduced the expression and secretion levels of VEGF in ARPE‑19 cells under hypoxic conditions. TTP destabilized the VEGF mRNA by binding to adenosine and uridine‑rich elements regions in its 3'‑untranslated region. Furthermore, conditioned medium (CM) from TTP‑overexpressing ARPE‑19 cells suppressed the tube formation in human umbilical vein endothelial cells compared with hypoxic CM. These findings indicate that regulation of TTP expression may be a promising therapeutic tool for neovascular AMD, however, further research is required.
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December-2016
Volume 14 Issue 6

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Ryu J, Seong H, Yoon NA, Seo SW, Park JW, Kang SS, Park JM and Han YS: Tristetraprolin regulates the decay of the hypoxia-induced vascular endothelial growth factor mRNA in ARPE-19 cells. Mol Med Rep 14: 5395-5400, 2016.
APA
Ryu, J., Seong, H., Yoon, N.A., Seo, S.W., Park, J.W., Kang, S.S. ... Han, Y.S. (2016). Tristetraprolin regulates the decay of the hypoxia-induced vascular endothelial growth factor mRNA in ARPE-19 cells. Molecular Medicine Reports, 14, 5395-5400. https://doi.org/10.3892/mmr.2016.5890
MLA
Ryu, J., Seong, H., Yoon, N. A., Seo, S. W., Park, J. W., Kang, S. S., Park, J. M., Han, Y. S."Tristetraprolin regulates the decay of the hypoxia-induced vascular endothelial growth factor mRNA in ARPE-19 cells". Molecular Medicine Reports 14.6 (2016): 5395-5400.
Chicago
Ryu, J., Seong, H., Yoon, N. A., Seo, S. W., Park, J. W., Kang, S. S., Park, J. M., Han, Y. S."Tristetraprolin regulates the decay of the hypoxia-induced vascular endothelial growth factor mRNA in ARPE-19 cells". Molecular Medicine Reports 14, no. 6 (2016): 5395-5400. https://doi.org/10.3892/mmr.2016.5890