Open Access

Carnosol inhibits osteoclastogenesis <em>in vivo</em> and <em>in vitro</em> by blocking the RANKL‑induced NF‑κB signaling pathway

Retraction in: /10.3892/mmr.2024.13369

  • Authors:
    • Pan Cai
    • Shichang Yan
    • Yan Lu
    • Xiaoxiao Zhou
    • Xiuhui Wang
    • Minghui Wang
    • Zhifeng Yin
  • View Affiliations

  • Published online on: May 19, 2022     https://doi.org/10.3892/mmr.2022.12741
  • Article Number: 225
  • Copyright: © Cai et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Bone homeostasis is maintained by osteoclast-mediated bone resorption and osteoblast‑mediated bone formation. Disruption of bone homeostasis due to excessive osteoclastogenesis or reduced osteogenesis results in various disorders, such as postmenopausal osteoporosis. Receptor activator of NF‑κB ligand (RANKL) stimulation of the NF‑κB signaling pathway is essential in osteoclastogenesis. The aim of the present study was to investigate the novel effects of carnosol, an active compound found in Rosmarinus officinalis, on RANKL‑induced osteoclastogenesis both in vitro and in vivo. TRAP staining showed that carnosol significantly inhibited osteoclasts differentiation of bone marrow monocytes and RAW264.7 cells. Western blot results showed that the protein expression levels of osteoclastogenesis‑associated genes, including cathepsin K, tartrate‑resistant acid phosphatase and MMP‑9, were markedly inhibited by carnosol, which may have suppressed osteoclast function. Furthermore, western blot and immunofluorescent staining results revealed that carnosol markedly suppressed the phosphorylation of p65 induced by RANKL and blocked its nuclear translocation, indicating the suppression of NF‑κB signaling pathway. H&E staining and micro‑CT results showed that in vivo treatment with carnosol significantly attenuated ovariectomy‑induced bone loss in mice. In conclusion, the present study indicated that carnosol may suppress osteoclastogenesis both in vivo and in vitro by inhibiting the activation of the NF‑κB signaling pathway. Carnosol may therefore be a potential novel therapeutic candidate for the clinical treatment of osteoclast‑related disorders.
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July-2022
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Spandidos Publications style
Cai P, Yan S, Lu Y, Zhou X, Wang X, Wang M and Yin Z: Carnosol inhibits osteoclastogenesis <em>in vivo</em> and <em>in vitro</em> by blocking the RANKL‑induced NF‑κB signaling pathway Retraction in /10.3892/mmr.2024.13369. Mol Med Rep 26: 225, 2022.
APA
Cai, P., Yan, S., Lu, Y., Zhou, X., Wang, X., Wang, M., & Yin, Z. (2022). Carnosol inhibits osteoclastogenesis <em>in vivo</em> and <em>in vitro</em> by blocking the RANKL‑induced NF‑κB signaling pathway Retraction in /10.3892/mmr.2024.13369. Molecular Medicine Reports, 26, 225. https://doi.org/10.3892/mmr.2022.12741
MLA
Cai, P., Yan, S., Lu, Y., Zhou, X., Wang, X., Wang, M., Yin, Z."Carnosol inhibits osteoclastogenesis <em>in vivo</em> and <em>in vitro</em> by blocking the RANKL‑induced NF‑κB signaling pathway Retraction in /10.3892/mmr.2024.13369". Molecular Medicine Reports 26.1 (2022): 225.
Chicago
Cai, P., Yan, S., Lu, Y., Zhou, X., Wang, X., Wang, M., Yin, Z."Carnosol inhibits osteoclastogenesis <em>in vivo</em> and <em>in vitro</em> by blocking the RANKL‑induced NF‑κB signaling pathway Retraction in /10.3892/mmr.2024.13369". Molecular Medicine Reports 26, no. 1 (2022): 225. https://doi.org/10.3892/mmr.2022.12741