LncRNA BX357664 inhibits cell proliferation and invasion and promotes cell apoptosis in human colorectal cancer cells

Liu,Fengjun; Wang,Xinsheng; Liu,Haiyan; Wang,Yang; Liu,Xiaoqian; Hao,Xiaochen; Li,Hongguang

doi:10.3892/ol.2018.8435

LncRNA BX357664 inhibits cell proliferation and invasion and promotes cell apoptosis in human colorectal cancer cells

Authors:
- Fengjun Liu
- Xinsheng Wang
- Haiyan Liu
- Yang Wang
- Xiaoqian Liu
- Xiaochen Hao
- Hongguang Li
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Affiliations: Department of General Surgery, Shandong University Qilu Hospital, Jinan, Shandong 250012, P.R. China, Department of Rehabilitation, Anqiu Maternal and Child Health Care Hospital, Anqiu, Shandong 262100, P.R. China, Department of General Surgery, Weifang People's Hospital, Weifang, Shandong 261000, P.R. China, Department of Obstetrics, Anqiu Maternal and Child Health Care Hospital, Anqiu, Shandong 262100, P.R. China, Department of Internal Medicine Cardiovascular, Anqiu People's Hospital, Anqiu, Shandong 262100, P.R. China, Department of Urology Surgery, Anqiu People's Hospital, Anqiu, Shandong 262100, P.R. China
Published online on: April 5, 2018 https://doi.org/10.3892/ol.2018.8435
Pages: 8237-8244
Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Colorectal cancer represents a great burden for patients worldwide. Long noncoding RNA BX357664 is an RNA that was identified by microarray technique in renal cell carcinoma. The function of BX357664 in solid tumors remains largely unknown. The present study aimed to investigate the expression profile and functional role of BX357664 in human colorectal cancer progression. The transcription levels of BX357664 were initially examined in vivo and in vitro. An overexpression plasmid was constructed in order to examine the effects of BX357664 overexpression on cell proliferation, apoptosis, migration and invasion. The results demonstrated that BX357664 was significantly downregulated in clinical colorectal cancer tissues and cell lines. Overexpression of BX357664 decreased cell proliferation rates and cell colony formation capacities in HCT116 and HT‑29 cells. Following BX357664 overexpression, HCT116 and HT‑29 cells exhibited reduced migration and invasion capacities. Would closure was also blunted by >50% following overexpression of BX357664 in HCT‑116 and HT‑29 cells. In addition, the cell cycle regulators Cyclin B1, CDC25C and Cyclin D1 as well as the mesenchymal marker N‑cadherin were downregulated, whereas the epithelial marker E‑cadherin was upregulated by BX357664 overexpression. Finally, HCT116 and HT‑29 cell apoptosis was induced and activities of caspase‑3 and caspase‑9 increased significantly following BX357664 overexpression. The present data suggested that BX257664 negatively regulated cell proliferation and metastasis and promoted cell apoptosis in colorectal cancer. These observations provided novel evidence that BX357664 might serve as a tumor suppressor and a potential therapeutic target in the treatment of colorectal cancer in the clinic.

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1	Ferlay J, Parkin DM and Steliarova-Foucher E: Estimates of cancer incidence and mortality in Europe in 2008. Eur J Cancer. 46:765–781. 2010. View Article : Google Scholar : PubMed/NCBI
2	Bray F, Ren JS, Masuyer E and Ferlay J: Global estimates of cancer prevalence for 27 sites in the adult population in 2008. Int J Cancer. 132:1133–1145. 2013. View Article : Google Scholar : PubMed/NCBI
3	Torre LA, Bray F, Siegel RL, Ferlay J, Lortet-Tieulent J and Jemal A: Global cancer statistics, 2012. CA Cancer J Clin. 65:87–108. 2015. View Article : Google Scholar : PubMed/NCBI
4	Guo P, Huang ZL, Yu P and Li K: Trends in cancer mortality in China: An update. Ann Oncol. 23:2755–2762. 2012. View Article : Google Scholar : PubMed/NCBI
5	Brenner H, Kloor M and Pox CP: Colorectal cancer. Lancet. 383:1490–1502. 2014. View Article : Google Scholar : PubMed/NCBI
6	de Hoon M, Shin JW and Carninci P: Paradigm shifts in genomics through the FANTOM projects. Mamm Genome. 26:391–402. 2015. View Article : Google Scholar : PubMed/NCBI
7	Martens-Uzunova ES, Bottcher R, Croce CM, Jenster G, Visakorpi T and Calin GA: Long noncoding RNA in prostate, bladder and kidney cancer. Eur Urol. 65:1140–1151. 2014. View Article : Google Scholar : PubMed/NCBI
8	Chen L, Wang W, Cao L, Li Z and Wang X: Long Non-Coding RNA CCAT1 acts as a competing endogenous RNA to regulate cell growth and differentiation in acute myeloid leukemia. Mol Cells. 39:330–336. 2016. View Article : Google Scholar : PubMed/NCBI
9	Huang FT, Chen WY, Gu ZQ, Zhuang YY, Li CQ, Wang LY, Peng JF, Zhu Z, Luo X, Li YH, et al: The novel long intergenic noncoding RNA UCC promotes colorectal cancer progression by sponging miR-143. Cell Death Dis. 8:e27782017. View Article : Google Scholar : PubMed/NCBI
10	Qin C, Han Z, Qian J, Bao M, Li P, Ju X, Zhang S, Zhang L, Li S, Cao Q, et al: Expression pattern of long non-coding RNAs in renal cell carcinoma revealed by microarray. PLoS One. 9:e993722014. View Article : Google Scholar : PubMed/NCBI
11	Liu Y, Qian J, Li X, Chen W, Xu A, Zhao K, Hua Y, Huang Z, Zhang J, Liang C, et al: Long noncoding RNA BX357664 regulates cell proliferation and epithelial-to-mesenchymal transition via inhibition of TGF-β1/p38/HSP27 signaling in renal cell carcinoma. Oncotarget. 7:81410–81422. 2016.PubMed/NCBI
12	Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI
13	Tomlinson JS, Jarnagin WR, DeMatteo RP, Fong Y, Kornprat P, Gonen M, Kemeny N, Brennan MF, Blumgart LH and D'Angelica M: Actual 10-year survival after resection of colorectal liver metastases defines cure. J Clin Oncol. 25:4575–4580. 2007. View Article : Google Scholar : PubMed/NCBI
14	Abdalla EK, Vauthey JN, Ellis LM, Ellis V, Pollock R, Broglio KR, Hess K and Curley SA: Recurrence and outcomes following hepatic resection, radiofrequency ablation and combined resection/ablation for colorectal liver metastases. Ann Surg. 239:818–827. 2004. View Article : Google Scholar : PubMed/NCBI
15	Li L and Luo HS: G-Protein signaling protein-17 (RGS17) is upregulated and promotes tumor growth and migration in human colorectal carcinoma. Oncol Res. 2017.(Epub ahead of print).
16	Prensner JR, Iyer MK, Balbin OA, Dhanasekaran SM, Cao Q, Brenner JC, Laxman B, Asangani IA, Grasso CS, Kominsky HD, et al: Transcriptome sequencing across a prostate cancer cohort identifies PCAT-1, an unannotated lincRNA implicated in disease progression. Nat Biotechnol. 29:742–749. 2011. View Article : Google Scholar : PubMed/NCBI
17	Li H, Ma SQ, Huang J, Chen XP and Zhou HH: Roles of long noncoding RNAs in colorectal cancer metastasis. Oncotarget. 8:39859–39876. 2017.PubMed/NCBI
18	Tao Y, Han T, Zhang T, Ma C and Sun C: LncRNA CHRF-induced miR-489 loss promotes metastasis of colorectal cancer via TWIST1/EMT signaling pathway. Oncotarget. 30:36410–36422. 2017.
19	Xu J, Zhang R and Zhao J: The novel long noncoding RNA TUSC7 inhibits proliferation by sponging MiR-211 in colorectal cancer. Cell Physiol Biochem. 41:635–644. 2017. View Article : Google Scholar : PubMed/NCBI
20	Wang L, Park HJ, Dasari S, Wang S, Kocher JP and Li W: CPAT: Coding-Potential assessment tool using an alignment-free logistic regression model. Nucleic Acids Res. 41:e742013. View Article : Google Scholar : PubMed/NCBI
21	Sherr CJ: Cancer cell cycles. Science. 274:1672–1677. 1996. View Article : Google Scholar : PubMed/NCBI
22	Nieto MA, Huang RY, Jackson RA and Thiery JP: Emt: 2016. Cell. 166:21–45. 2016. View Article : Google Scholar : PubMed/NCBI
23	Xu XY, Xia P, Yu M, Nie XC, Yang X, Xing YN, Liu YP, Takano Y and Zheng HC: The roles of REIC gene and its encoding product in gastric carcinoma. Cell Cycle. 11:1414–1431. 2012. View Article : Google Scholar : PubMed/NCBI
24	Du Y, Gong J, Tian X, Yan X, Guo T, Huang M, Zhang B, Hu X, Liu H, Wang Y, et al: Japonicone a inhibits the growth of non-small cell lung cancer cells via mitochondria-mediated pathways. Tumour Biol. 36:7473–7482. 2015. View Article : Google Scholar : PubMed/NCBI