Open Access

OTUD7B knockdown inhibits the proliferation and stemness of breast cancer cells by destabilizing FOXM1

  • Authors:
    • Hebing Wang
    • Sumei Han
    • Jian Xiao
    • Xinghang Fu
    • Wenfeng Chen
    • Dexiang Zhuo
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  • Published online on: January 16, 2024     https://doi.org/10.3892/ol.2024.14235
  • Article Number: 102
  • Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Breast cancer is a leading cause of cancer‑related death in women worldwide; therefore, there is an urgent need to develop novel therapies and drugs that prolong the survival and improve the quality of life of patients with breast cancer. In the present study, the effects and underlying mechanisms of OTU domain‑containing 7B (OTUD7B) knockdown on breast cancer were investigated using MDA‑MB‑468, MDA‑MB‑453 and MCF7 cell lines. The results of Cell Counting Kit 8, colony formation and tumor sphere formation experiments showed that OTUD7B knockdown caused a significant decrease in the proliferation and sphere formation ability of MDA‑MB‑468, MDA‑MB‑453 and MCF7 cells in vitro. Moreover, western blotting results showed that CD44, EpCAM, SOX2 and Nanog protein levels were significantly decreased following OTUD7B knockdown. These findings indicated that OTUD7B knockdown reduced the proliferation and stemness of breast cancer cells. Co‑immunoprecipitation assays demonstrated that OTUD7B interacted with forkhead box protein M1 (FOXM1) and reduced the polyubiquitylation of FOXM1 in breast cancer cells; accordingly, FOXM1 protein levels were significantly decreased by OTUD7B knockdown. Furthermore, the overexpression of FOXM1 reduced the inhibitory effects of OTUD7B knockdown on breast cancer cells. The findings of the present study provide new insights into the oncogenic role of OTUD7B in breast cancer and indicate that OTUD7B may serve as a therapeutic target for breast cancer.
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March-2024
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Copy and paste a formatted citation
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Spandidos Publications style
Wang H, Han S, Xiao J, Fu X, Chen W and Zhuo D: OTUD7B knockdown inhibits the proliferation and stemness of breast cancer cells by destabilizing FOXM1. Oncol Lett 27: 102, 2024
APA
Wang, H., Han, S., Xiao, J., Fu, X., Chen, W., & Zhuo, D. (2024). OTUD7B knockdown inhibits the proliferation and stemness of breast cancer cells by destabilizing FOXM1. Oncology Letters, 27, 102. https://doi.org/10.3892/ol.2024.14235
MLA
Wang, H., Han, S., Xiao, J., Fu, X., Chen, W., Zhuo, D."OTUD7B knockdown inhibits the proliferation and stemness of breast cancer cells by destabilizing FOXM1". Oncology Letters 27.3 (2024): 102.
Chicago
Wang, H., Han, S., Xiao, J., Fu, X., Chen, W., Zhuo, D."OTUD7B knockdown inhibits the proliferation and stemness of breast cancer cells by destabilizing FOXM1". Oncology Letters 27, no. 3 (2024): 102. https://doi.org/10.3892/ol.2024.14235