Association of ADP‑ribosylation factor family genes with prognosis and immune infiltration of breast cancer

Yang,Lixian; Zhang,Shiyu; Zheng,Lei; Kong,Fanting; Pu,Pengpeng; Li,Xiaowei; Jia,Lining

doi:10.3892/ol.2024.14413

Association of ADP‑ribosylation factor family genes with prognosis and immune infiltration of breast cancer

Authors:
- Lixian Yang
- Shiyu Zhang
- Lei Zheng
- Fanting Kong
- Pengpeng Pu
- Xiaowei Li
- Lining Jia
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Affiliations: Department of Breast Surgery, Xingtai People's Hospital, Xingtai, Hebei 054000, P.R. China
Published online on: April 23, 2024 https://doi.org/10.3892/ol.2024.14413
Article Number: 280
Copyright: © Yang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Breast cancer (BC) is the most common type of cancer found in women. ADP‑ribosylation factors (ARFs) are a group of small proteins that bind to GTP and are involved in controlling different cellular functions. The function and evolution of multiple ARFs in BC have remained to be fully elucidated, despite existing studies on this protein family in Homo sapiens and other species. In the present study, a systematic analysis of ARF expression levels in BC tissues compared to normal breast tissues was performed using data from The Cancer Genome Atlas database. The analysis revealed significantly higher expression of ARFs in BC tissues. In addition, the prognostic significance of ARF1 and ARF3‑6 expression levels was assessed in patients with BC. Of note, elevated ARF1 expression was associated with reduced rates of distant metastasis‑free survival (DMFS), overall survival (OS) and recurrence‑free survival (RFS) in affected individuals. Similarly, patients with high expression levels of ARF3 had lower post‑progression survival (PPS) rates. In addition, patients with higher ARF4 expression had worse PPS and patients with high ARF5 expression exhibited lower DMFS. Patients with high ARF6 expression had worse DMFS, OS, RFS and predictive power score values. Furthermore, the expression of ARF was found to be strongly linked to the infiltration of various immune cell types, namely dendritic cells, macrophages, neutrophils, CD8+ T cells and B cells. These significant associations offer a solid foundation for the potential utilization of new therapeutic targets and predictive markers for the treatment of BC.

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