DIFFERENTIAL REGULATION OF GLUCOSE-TRANSPORTER EXPRESSION IN HEMATOPOIETIC-CELLS BY ONCOGENIC TRANSFORMATION AND CYTOKINE STIMULATION
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- Published online on: January 1, 1994 https://doi.org/10.3892/or.1.1.17
- Pages: 17-26
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Abstract
Malignant transformation by the v-src oncogene and mitogenic stimulation by interleukin-3 (IL-3) both increased glucose transport into a hematopoietic cell line. These increases were additive and correlated with elevations in the level of GLUT1 mRNA. Glucose transport and GLUT1 mRNA were dependent on the presence of a functional v-src gene product in the absence of IL-3. Nuclear run-on analyses and mRNA turnover experiments demonstrated that GLUT1 gene transcription was enhanced by v-src while IL-3 stabilized GLUT1 mRNA. Introduction of retroviruses overexpressing GLUT1 into factor-dependent cells did not abrogate factor-dependency. Thus, GLUT1 induction is necessary but not sufficient for mitogenesis.
