Identification of ABCG2+ cells in nasopharyngeal carcinoma cells

  • Authors:
    • Hongbo Zhang
    • Weidong Liu
    • Xiangling Feng
    • Lei Wang
    • Xingjun Jiang
    • Dingyang Liu
    • Lihua Zhang
    • Bin Zhu
    • Wen Zhou
    • Wenting Jia
    • Guifei Li
    • Caiping Ren
  • View Affiliations

  • Published online on: December 30, 2011     https://doi.org/10.3892/or.2011.1618
  • Pages: 1177-1187
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Abstract

Tumor stem cells are a small subset of tumor cells with the ability of self-renewal and differentiation and are regarded as a cause of tumor growth and recurrence. Previously we have shown that stem-like label-retaining cells (LRCs) can be detected in nasopharynx, tongue, esophagus and xenograft tumors formed by nasopharyngeal carcinoma (NPC) cell lines (5-8F, 6-10B and TMNE). The present study aimed to identify ABCG2+ cells in 5-8F NPC cells and compare their tumorigenic potential with ABCG2- cells, expecting that we can obtain insight into the mechanism of the differential phenotypes of ABCG2+ and ABCG2- cells. By using magnetic cell sorting (MACS) method, we isolated ABCG2+ cells and ABCG2- cells from 5-8F cells. Among these two subpopulations and unsorted 5-8F cells, the rate of ABCG2+ cells at G1 phase was highest, while the rate of ABCG2- cells at S phase was highest, indicating that ABCG2+ cells were mostly quiescent. However, ABCG2+ cells showed lower cloning efficiency and tumorigenicity than ABCG2- cells. We also used Affymetrix U133 plus 2.0 human whole genome expression chip to identify the gene expression profile of ABCG2+ and ABCG2- cells and found that both subpopulations expressed some stem cell associated genes, e.g., PSCA, ABCG2 and ALPI were expressed in ABCG2+ cells, and K19, integrin α6, integrin β4, CD44 and K14 were expressed in ABCG2- cells, suggesting there were stem cells in both ABCG2+ and ABCG2- cells. Our data demonstrated that there exist ABCG2+ cells in NPC cells, but ABCG2 alone is not sufficient for isolating cancer stem cells in 5-8F NPC cells.
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April 2012
Volume 27 Issue 4

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Zhang H, Liu W, Feng X, Wang L, Jiang X, Liu D, Zhang L, Zhu B, Zhou W, Jia W, Jia W, et al: Identification of ABCG2+ cells in nasopharyngeal carcinoma cells. Oncol Rep 27: 1177-1187, 2012
APA
Zhang, H., Liu, W., Feng, X., Wang, L., Jiang, X., Liu, D. ... Ren, C. (2012). Identification of ABCG2+ cells in nasopharyngeal carcinoma cells. Oncology Reports, 27, 1177-1187. https://doi.org/10.3892/or.2011.1618
MLA
Zhang, H., Liu, W., Feng, X., Wang, L., Jiang, X., Liu, D., Zhang, L., Zhu, B., Zhou, W., Jia, W., Li, G., Ren, C."Identification of ABCG2+ cells in nasopharyngeal carcinoma cells". Oncology Reports 27.4 (2012): 1177-1187.
Chicago
Zhang, H., Liu, W., Feng, X., Wang, L., Jiang, X., Liu, D., Zhang, L., Zhu, B., Zhou, W., Jia, W., Li, G., Ren, C."Identification of ABCG2+ cells in nasopharyngeal carcinoma cells". Oncology Reports 27, no. 4 (2012): 1177-1187. https://doi.org/10.3892/or.2011.1618