Casticin, a flavonoid, potentiates TRAIL-induced apoptosis through modulation of anti-apoptotic proteins and death receptor 5 in colon cancer cells

  • Authors:
    • San-Yuan Tang
    • Mei-Zuo Zhong
    • Guang-Jin Yuan
    • Su-Ping Hou
    • Lei-Lan Yin
    • Hao Jiang
    • Zheng-Υang Yu
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  • Published online on: November 7, 2012     https://doi.org/10.3892/or.2012.2127
  • Pages: 474-480
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Abstract

We investigated the effect of casticin on apoptosis induced by tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL). We found that casticin potentiated TRAIL-induced apoptosis in human colon cancer cells. Casticin downregulated cell survival proteins including Bcl-xL, Bcl-2, survivin, XIAP and cFLIP, and induced death receptor 5 (DR5), but had no effect on DR4 and decoy receptors (DcR1 or DcR2). Deletion of DR5 by siRNA significantly reduced the apoptosis induced by TRAIL and casticin. In addition, casticin induced reactive oxygen species (ROS) generation in a dose-dependent manner. Collectively, the present study showed that casticin potentiates TRAIL-induced apoptosis through downregulation of cell survival proteins and induction of DR5 mediated by ROS.
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February 2013
Volume 29 Issue 2

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Tang S, Zhong M, Yuan G, Hou S, Yin L, Jiang H and Yu Z: Casticin, a flavonoid, potentiates TRAIL-induced apoptosis through modulation of anti-apoptotic proteins and death receptor 5 in colon cancer cells. Oncol Rep 29: 474-480, 2013
APA
Tang, S., Zhong, M., Yuan, G., Hou, S., Yin, L., Jiang, H., & Yu, Z. (2013). Casticin, a flavonoid, potentiates TRAIL-induced apoptosis through modulation of anti-apoptotic proteins and death receptor 5 in colon cancer cells. Oncology Reports, 29, 474-480. https://doi.org/10.3892/or.2012.2127
MLA
Tang, S., Zhong, M., Yuan, G., Hou, S., Yin, L., Jiang, H., Yu, Z."Casticin, a flavonoid, potentiates TRAIL-induced apoptosis through modulation of anti-apoptotic proteins and death receptor 5 in colon cancer cells". Oncology Reports 29.2 (2013): 474-480.
Chicago
Tang, S., Zhong, M., Yuan, G., Hou, S., Yin, L., Jiang, H., Yu, Z."Casticin, a flavonoid, potentiates TRAIL-induced apoptosis through modulation of anti-apoptotic proteins and death receptor 5 in colon cancer cells". Oncology Reports 29, no. 2 (2013): 474-480. https://doi.org/10.3892/or.2012.2127