Acquisition of paclitaxel resistance via PI3K‑dependent epithelial‑mesenchymal transition in A2780 human ovarian cancer cells

Du,Fangfang; Wu,Xiaohong; Liu,Yanjun; Wang,Teng; Qi,Xiaowei; Mao,Yong; Jiang,Li; Zhu,Yifei; Chen,Yun; Zhu,Ruiyu; Han,Xiaofeng; Jin,Jian; Ma,Xin; Hua,Dong

doi:10.3892/or.2013.2567

Acquisition of paclitaxel resistance via PI3K‑dependent epithelial‑mesenchymal transition in A2780 human ovarian cancer cells

Authors:
- Fangfang Du
- Xiaohong Wu
- Yanjun Liu
- Teng Wang
- Xiaowei Qi
- Yong Mao
- Li Jiang
- Yifei Zhu
- Yun Chen
- Ruiyu Zhu
- Xiaofeng Han
- Jian Jin
- Xin Ma
- Dong Hua
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Affiliations: School of Pharmaceutical Sciences, Jiangnan University, Wuxi, Jiangsu, P.R. China, Affiliated Hospital, Jiangnan University, Wuxi, Jiangsu, P.R China, School of Medicine, Jiangnan University, Wuxi, Jiangsu, P.R. China
Published online on: June 26, 2013 https://doi.org/10.3892/or.2013.2567
Pages: 1113-1118

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Abstract

Epithelial ovarian cancer is a major cause of mortality among women with gynecological malignancies. Paclitaxel is commonly used for chemotherapy of ovarian cancer, yet its efficacy is limited by chemoresistance. Generally, drug resistance is associated with acquisition of the epithelial-mesenchymal transition (EMT) in cancer. The aim of the present study was to determine whether the EMT is involved in acquired resistance to paclitaxel in A2780 human ovarian cancer cells. Using the paclitaxel-resistant A2780/PTX cell line, we examined the cellular morphology, molecular changes, migration and proliferation consistent with the EMT. Furthermore, we found that inhibition of phosphatidylinositol 3-kinase (PI3K) activity reduced the proliferation and migration and restored their sensitivity to paclitaxel. Our study provides new insights into EMT-like phenotypic changes that are linked to paclitaxel resistance in A2780 cells. We believe that inhibition of the PI3K signaling pathway could provide a novel therapeutic approach to overcome chemoresistance and prevent metastasis during paclitaxel chemotherapy.

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