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Article

Decreased c-Abl activity in PC-3 and LNCaP prostate cancer cells overexpressing the early growth response-1 protein

  • Authors:
    • Eduardo Parra
    • Jorge Ferreira
    • Luis Gutierrez
  • View Affiliations / Copyright

    Affiliations: Laboratory of Experimental Biomedicine, University of Tarapaca, Campus Esmeralda, Iquique, Chile, Programme of Molecular and Clinical Pharmacology, ICBM, Medical Faculty, University of Chile, Santiago, Chile, Faculty of Sciences, Arturo Prat University, Iquique, Chile
  • Pages: 422-427
    |
    Published online on: October 31, 2013
       https://doi.org/10.3892/or.2013.2829
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Abstract

Early growth response-1 (Egr-1) and the non‑receptor protein tyrosine kinase (c-Abl) are 2 response genes that can act as regulators of cell growth and apoptosis in response to stress. Both Egr-1 and c-Abl regulate cell proliferation and survival in different types of cancer cells. To study the effect of overexpression of EGR-1 on the activity of c-Abl in prostate cancer cells, human PC-3 and LNCaP cells were transfected with a control vector or a vector containing the murine Egr-1 cDNA and assessed for the expression of the c-Abl gene. Cells overexpressing Egr-1 were studied with respect to apoptosis (Annexin V)/DEVDase activity, Egr-1/c-Abl activation (western blotting) and cell proliferation (MTT assay). The cells were exposed to tumor necrosis factor α (TNF-α), a known inductor of Egr-1, to c-Abl inhibitor STI-571 and to small interfering RNA (siRNA)-Egr-1, respectively. The results from our studies strongly suggest that overexpression of Egr-1 decreased c-Abl activity independent of endogenous Egr-1 inhibition by siRNA-Egr-1.
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Copy and paste a formatted citation
Spandidos Publications style
Parra E, Ferreira J and Gutierrez L: Decreased c-Abl activity in PC-3 and LNCaP prostate cancer cells overexpressing the early growth response-1 protein. Oncol Rep 31: 422-427, 2014.
APA
Parra, E., Ferreira, J., & Gutierrez, L. (2014). Decreased c-Abl activity in PC-3 and LNCaP prostate cancer cells overexpressing the early growth response-1 protein. Oncology Reports, 31, 422-427. https://doi.org/10.3892/or.2013.2829
MLA
Parra, E., Ferreira, J., Gutierrez, L."Decreased c-Abl activity in PC-3 and LNCaP prostate cancer cells overexpressing the early growth response-1 protein". Oncology Reports 31.1 (2014): 422-427.
Chicago
Parra, E., Ferreira, J., Gutierrez, L."Decreased c-Abl activity in PC-3 and LNCaP prostate cancer cells overexpressing the early growth response-1 protein". Oncology Reports 31, no. 1 (2014): 422-427. https://doi.org/10.3892/or.2013.2829
Copy and paste a formatted citation
x
Spandidos Publications style
Parra E, Ferreira J and Gutierrez L: Decreased c-Abl activity in PC-3 and LNCaP prostate cancer cells overexpressing the early growth response-1 protein. Oncol Rep 31: 422-427, 2014.
APA
Parra, E., Ferreira, J., & Gutierrez, L. (2014). Decreased c-Abl activity in PC-3 and LNCaP prostate cancer cells overexpressing the early growth response-1 protein. Oncology Reports, 31, 422-427. https://doi.org/10.3892/or.2013.2829
MLA
Parra, E., Ferreira, J., Gutierrez, L."Decreased c-Abl activity in PC-3 and LNCaP prostate cancer cells overexpressing the early growth response-1 protein". Oncology Reports 31.1 (2014): 422-427.
Chicago
Parra, E., Ferreira, J., Gutierrez, L."Decreased c-Abl activity in PC-3 and LNCaP prostate cancer cells overexpressing the early growth response-1 protein". Oncology Reports 31, no. 1 (2014): 422-427. https://doi.org/10.3892/or.2013.2829
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