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2014-January Volume 31 Issue 1

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International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

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Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

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Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

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Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

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Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

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Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

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Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

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International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

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Article

Sanguinarine inhibits invasiveness and the MMP‑9 and COX‑2 expression in TPA-induced breast cancer cells by inducing HO-1 expression

  • Authors:
    • Sun Young Park
    • Mei Ling Jin
    • Young Hun Kim
    • Sang-Joon Lee
    • Geuntae Park
  • View Affiliations / Copyright

    Affiliations: Bio-IT Fusion Technology Research Institute, Pusan National University, Busan 609-735, Republic of Korea, Department of Microbiology, Pusan National University, Busan 609-735, Republic of Korea, Institute for Research and Industry Cooperation, Pusan National University, Busan 609-735, Republic of Korea
  • Pages: 497-504
    |
    Published online on: November 12, 2013
       https://doi.org/10.3892/or.2013.2843
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Abstract

Most complications of breast cancer are attributed to metastasis to distant organs, including lymph nodes, bone, lung and liver. Metastasis is considered the leading cause of mortality in patients with breast cancer. The emergence of anti-metastatic properties in breast cancer is an important clinical phenomenon affecting long-term survival. In the present study, we investigated the anti-invasive mechanism of sanguinarine by focusing on its role in inducing HO-1 in breast cancer cells. The results showed that sanguinarine inhibited TPA-induced MMP-9 and COX‑2 mRNA and protein expression in a dose-dependent manner at non-cytotoxic concentrations. Similarly, the MMP-9 enzymatic activity and the PGE2 levels significantly decreased in MCF-7 breast cancer cells. TIMP‑1 and TIMP-2, specific endogenous inhibitors of MMP-9, were slightly induced by sanguinarine. Subsequent studies revealed that sanguinarine suppressed TPA-induced NF-κB and AP-1 activation, as well as the phosphorylation of Akt and ERK. Furthermore, sanguinarine significantly inhibited TPA-induced invasion and migration in breast cancer cells. We also demonstrated that sanguinarine induced HO-1 expression, and that the inhibition of MMP-9 and COX-2 expression and the enzymatic activity of sanguinarine were abrogated by siRNA-mediated knockdown of HO-1 expression. Thus, knockdown of endogenous HO-1 decreased TPA-induced cell invasion. Overall, the results of the present study demonstrate that HO-1 plays a pivotal role in the anti-invasive response of sanguinarine in TPA-stimulated breast cancer cells.
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Copy and paste a formatted citation
Spandidos Publications style
Park SY, Jin ML, Kim YH, Lee S and Park G: Sanguinarine inhibits invasiveness and the MMP‑9 and COX‑2 expression in TPA-induced breast cancer cells by inducing HO-1 expression. Oncol Rep 31: 497-504, 2014.
APA
Park, S.Y., Jin, M.L., Kim, Y.H., Lee, S., & Park, G. (2014). Sanguinarine inhibits invasiveness and the MMP‑9 and COX‑2 expression in TPA-induced breast cancer cells by inducing HO-1 expression. Oncology Reports, 31, 497-504. https://doi.org/10.3892/or.2013.2843
MLA
Park, S. Y., Jin, M. L., Kim, Y. H., Lee, S., Park, G."Sanguinarine inhibits invasiveness and the MMP‑9 and COX‑2 expression in TPA-induced breast cancer cells by inducing HO-1 expression". Oncology Reports 31.1 (2014): 497-504.
Chicago
Park, S. Y., Jin, M. L., Kim, Y. H., Lee, S., Park, G."Sanguinarine inhibits invasiveness and the MMP‑9 and COX‑2 expression in TPA-induced breast cancer cells by inducing HO-1 expression". Oncology Reports 31, no. 1 (2014): 497-504. https://doi.org/10.3892/or.2013.2843
Copy and paste a formatted citation
x
Spandidos Publications style
Park SY, Jin ML, Kim YH, Lee S and Park G: Sanguinarine inhibits invasiveness and the MMP‑9 and COX‑2 expression in TPA-induced breast cancer cells by inducing HO-1 expression. Oncol Rep 31: 497-504, 2014.
APA
Park, S.Y., Jin, M.L., Kim, Y.H., Lee, S., & Park, G. (2014). Sanguinarine inhibits invasiveness and the MMP‑9 and COX‑2 expression in TPA-induced breast cancer cells by inducing HO-1 expression. Oncology Reports, 31, 497-504. https://doi.org/10.3892/or.2013.2843
MLA
Park, S. Y., Jin, M. L., Kim, Y. H., Lee, S., Park, G."Sanguinarine inhibits invasiveness and the MMP‑9 and COX‑2 expression in TPA-induced breast cancer cells by inducing HO-1 expression". Oncology Reports 31.1 (2014): 497-504.
Chicago
Park, S. Y., Jin, M. L., Kim, Y. H., Lee, S., Park, G."Sanguinarine inhibits invasiveness and the MMP‑9 and COX‑2 expression in TPA-induced breast cancer cells by inducing HO-1 expression". Oncology Reports 31, no. 1 (2014): 497-504. https://doi.org/10.3892/or.2013.2843
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