Stromal fibroblasts are predictors of disease-related mortality in esophageal squamous cell carcinoma

Saito,Shin; Morishima,Kazue; Ui,Takashi; Matsubara,Daisuke; Tamura,Tomoko; Oguni,Sachiko; Hosoya,Yoshinori; Sata,Naohiro; Lefor,Alan  T.; Yasuda,Yoshikazu; Niki,Toshiro

doi:10.3892/or.2014.3216

Stromal fibroblasts are predictors of disease-related mortality in esophageal squamous cell carcinoma

Authors:
- Shin Saito
- Kazue Morishima
- Takashi Ui
- Daisuke Matsubara
- Tomoko Tamura
- Sachiko Oguni
- Yoshinori Hosoya
- Naohiro Sata
- Alan T. Lefor
- Yoshikazu Yasuda
- Toshiro Niki
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Affiliations: Department of Surgery, Jichi Medical University, Shimotsuke-City, Tochigi 329-0498, Japan, Department of Integrative Pathology, Jichi Medical University, Shimotsuke-City, Tochigi 329-0498, Japan
Published online on: May 23, 2014 https://doi.org/10.3892/or.2014.3216
Pages: 348-354

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Abstract

The growth, invasiveness and metastasis of human cancers are determined not only by cancer cells, but also by their microenvironment. Activated stromal fibroblasts promote tumor progression by secreting growth factors. In the present study, we focused on interrelations between cancer and fibroblasts, the main component of tumor stroma. We retrospectively analyzed the relations of mortality to clinical, pathological, and α-smooth muscle actin (α-SMA) characteristics in 97 consecutive patients with esophageal squamous cell carcinoma (ESCC). In vitro, we used TE-11, KYSE150 and KYSE220 ESCC cell lines and isolated esophageal stromal fibroblasts, some of which were immortalized. Migration assays were conducted to assess the effects of fibroblasts on cancer-cell migration and 3-dimensional organotypic cultures. In vivo, TE-11 and KYSE220 cells plus immortalized fibroblasts were co-transplanted subcutaneously in Nod/Scid mice to assess the effects of fibroblasts on tumorigenicity. Clinicopathologically, the α-SMA expression of cancer stroma was correlated with venous invasion (p<0.01), nodal involvement (p=0.02), recurrence (p=0.01), and was a predictor of survival in patients with stage I and II ESCC (p=0.04). In vitro, the presence of fibroblasts strongly promoted the migration of TE-11, KYSE150 and KYSE220 cells. On organotypic culture, stromal invasion was observed only in the presence of immortalized fibroblasts. In vivo, tumors developed or grew in a fibroblast‑dependent manner after implantation. Our findings provide evidence that stromal fibroblasts and tumor cells interact to promote tumor progression in ESCC. In patients with earlier stage ESCC, α-SMA may be a predictor of mortality. Inhibition of paracrine systems associated with tumor fibroblasts may slow or reverse tumor progression, potentially leading to the development of new targeted therapies.

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