Integrated analysis of differentially expressed mRNAs and miRNAs between hepatocellular carcinoma and their matched adjacent normal liver tissues

  • Authors:
    • Bing Gao
    • Shufang Ning
    • Jilin Li
    • Haizhou Liu
    • Wene Wei
    • Feixiang Wu
    • Yanping Tang
    • Yan Feng
    • Kezhi Li
    • Litu Zhang
  • View Affiliations

  • Published online on: May 8, 2015     https://doi.org/10.3892/or.2015.3968
  • Pages: 325-333
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Hepatocellular carcinoma has a high mortality rate, thus, there is a need for improvement of prognosis of such patients. The aim of the present study was to identify differentially expressed mRNAs and miRNAs between hepatocellular carcinoma tissues and their matched adjacent normal liver tissues, and to carry out a bioinformatics analysis. Agilent 8x60K microarray technology was used to detect the changes of mRNA and miRNA expression between hepatocellular carcinoma tissues and their matched adjacent normal liver tissues. To select differentially expressed mRNAs and miRNAs, gene ontology (GO) and pathway analysis were performed using bioinformatics methods. qPCR was used to verify the microarray data. As a result, 924 mRNAs and 21 miRNAs exhibited a higher expression in the hepatocellular carcinoma tissue than their matched adjacent normal liver tissue. In comparison with the adjacent normal tissue, the carcinoma tissue showed a downregulated expression of 1,770 mRNAs and 12 miRNAs. The GO and pathway analysis showed that these RNAs were involved in the transcription process, REDOX, signal transduction, ion transport, immune response, cell adhesion and binding functions. A total of 572 target genes of 14 miRNAs were identified, most of which were involved in tumors. The results of qPCR were in concordance with the microarray results. In summary, the differentially expressed mRNAs and miRNAs that include signal transduction, immune response and many other key links may provide novel targets for early diagnosis and therapy of hepatocellular carcinoma.
View Figures
View References

Related Articles

Journal Cover

July-2015
Volume 34 Issue 1

Print ISSN: 1021-335X
Online ISSN:1791-2431

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Gao B, Ning S, Li J, Liu H, Wei W, Wu F, Tang Y, Feng Y, Li K, Zhang L, Zhang L, et al: Integrated analysis of differentially expressed mRNAs and miRNAs between hepatocellular carcinoma and their matched adjacent normal liver tissues. Oncol Rep 34: 325-333, 2015
APA
Gao, B., Ning, S., Li, J., Liu, H., Wei, W., Wu, F. ... Zhang, L. (2015). Integrated analysis of differentially expressed mRNAs and miRNAs between hepatocellular carcinoma and their matched adjacent normal liver tissues. Oncology Reports, 34, 325-333. https://doi.org/10.3892/or.2015.3968
MLA
Gao, B., Ning, S., Li, J., Liu, H., Wei, W., Wu, F., Tang, Y., Feng, Y., Li, K., Zhang, L."Integrated analysis of differentially expressed mRNAs and miRNAs between hepatocellular carcinoma and their matched adjacent normal liver tissues". Oncology Reports 34.1 (2015): 325-333.
Chicago
Gao, B., Ning, S., Li, J., Liu, H., Wei, W., Wu, F., Tang, Y., Feng, Y., Li, K., Zhang, L."Integrated analysis of differentially expressed mRNAs and miRNAs between hepatocellular carcinoma and their matched adjacent normal liver tissues". Oncology Reports 34, no. 1 (2015): 325-333. https://doi.org/10.3892/or.2015.3968